izpis_h1_title_alt

Antibiotic resistance, virulence factors, phenotyping, and genotyping of E. coli isolated from the feces of healthy subjects
ID Raimondi, Stefano (Avtor), ID Righini, Lucia (Avtor), ID Candeliere, Francesco (Avtor), ID Musmeci, Eliana (Avtor), ID Bonvicini, Francesca (Avtor), ID Gentilomi, Giovanna (Avtor), ID Starčič Erjavec, Marjanca (Avtor), ID Amaretti, Alberto (Avtor), ID Rossi, Maddalena (Avtor)

.pdfPDF - Predstavitvena datoteka, prenos (3,19 MB)
MD5: E47AF68699A31F190EC5A3B06ED313A1
URLURL - Izvorni URL, za dostop obiščite https://www.mdpi.com/2076-2607/7/8/251 Povezava se odpre v novem oknu

Izvleček
Escherichia coli may innocuously colonize the intestine of healthy subjects or may instigate infections in the gut or in other districts. This study investigated intestinal E. coli isolated from 20 healthy adults. Fifty-one strains were genotyped by molecular fingerprinting and analyzed for genetic and phenotypic traits, encompassing the profile of antibiotic resistance, biofilm production, the presence of surface structures (such as curli and cellulose), and their performance as recipients in conjugation experiments. A phylogroup classification and analysis of 34 virulence determinants, together with genes associated to the pks island (polyketide-peptide genotoxin colibactin) and conjugative elements, was performed. Most of the strains belonged to the phylogroups B1 and B2. The different phylogroups were separated in a principal coordinate space, considering both genetic and functional features, but not considering pulsed-field gel electrophoresis. Within the B2 and F strains, 12 shared the pattern of virulence genes with potential uropathogens. Forty-nine strains were sensitive to all the tested antibiotics. Strains similar to the potential pathogens innocuously inhabited the gut of healthy subjects. However, they may potentially act as etiologic agents of extra-intestinal infections and are susceptible to a wide range of antibiotics. Nevertheless, there is still the possibility to control infections with antibiotic therapy.

Jezik:Angleški jezik
Ključne besede:Escherichia coli, typing, gut microbiota, PFGE, pulsed-field gel electrophoresis, virulence, antibiotic resistance, conjugation, curli, co-occurrence
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2019
Št. strani:18 str.
Številčenje:Vol. 7, iss. 8, art. 251
PID:20.500.12556/RUL-132789 Povezava se odpre v novem oknu
UDK:579
ISSN pri članku:2076-2607
DOI:10.3390/microorganisms7080251 Povezava se odpre v novem oknu
COBISS.SI-ID:5151311 Povezava se odpre v novem oknu
Datum objave v RUL:03.11.2021
Število ogledov:703
Število prenosov:127
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
:
Kopiraj citat
Objavi na:Bookmark and Share

Gradivo je del revije

Naslov:Microorganisms
Skrajšan naslov:Microorganisms
Založnik:MDPI AG
ISSN:2076-2607
COBISS.SI-ID:523277081 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:10.08.2019

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Nazaj