Screening of mosquitoes for West Nile virus and Usutu virus in Croatia, 2015–2020

Klobučar, Ana (avtor), Savić, Vladimir (avtor), Curman-Posavec, Marcela (avtor), Petrinic, Suncica (avtor), Kuhar, Urška (avtor), Toplak, Ivan (avtor), Madić, Josip (avtor), Vilibić-Čavlek, Tatjana (avtor)

In the period from 2015 to 2020, an entomological survey for the presence of West Nile virus (WNV) and Usutu virus (USUV) in mosquitoes was performed in northwestern Croatia. A total of 20,363 mosquitoes were sampled in the City of Zagreb and Međimurje county, grouped in 899 pools and tested by real-time RT-PCR for WNV and USUV RNA. All pools were negative for WNV while one pool each from 2016 (Aedes albopictus), 2017 (Culex pipiens complex), 2018 (Cx. pipiens complex), and 2019 (Cx. pipiens complex), respectively, was positive for USUV. The 2018 and 2019 positive pools shared 99.31% nucleotide homology within the USUV NS5 gene and both clustered within USUV Europe 2 lineage. The next-generation sequencing of one mosquito pool (Cx. pipiens complex) collected in 2018 in Zagreb confirmed the presence of USUV and revealed several dsDNA and ssRNA viruses of insect, bacterial and mammalian origin.

• Jezik: Angleški jezik
• Ključne besede: Usutu virus, West Nile virus, mosquitoes, Croatia, epidemiology, mosquito vectors
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 12 str.
• Številčenje: Vol. 6, iss. 2, art. 45
• PID: 20.500.12556/RUL-132322
• UDK: 636.09:616.9
• ISSN pri članku: 2414-6366
• DOI: 10.3390/tropicalmed6020045
• COBISS.SI-ID: 58396419
• Datum objave v RUL: 21.10.2021

Gradivo je del revije

• Naslov: Tropical medicine and infectious disease
• Skrajšan naslov: Trop. med. infect. dis.
• Založnik: MDPI
• ISSN: 2414-6366
• COBISS.SI-ID: 526463769

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.06.2021

Projekti

• Financer: HRZZ - Croatian Science Foundation
• Številka projekta: IP-2016-06-7456
• Naslov: Prevalence and molecular epidemiology of emerging and re-emerging neuroinvasive arboviral infections in Croatia
• Akronim: CRONEUROARBO