How accurate are veterinary clinicians employing flexicult vet for identification and antimicrobial susceptibility testing of urinary bacteria

Cugmas, Blaž (avtor), Avberšek, Miha (avtor), Rosa, Teja (avtor), Godec, Leonida (avtor), Struc, Eva (avtor), Golob, Majda (avtor), Zdovc, Irena (avtor)

Abstract Antibiotics are frequently used for treating urinary tract infections (UTI) in dogs and cats. UTI often requires time-consuming and expensive antimicrobial susceptibility testing (AST). Alternatively, clinicians can employ Flexicult Vet, an affordable chromogenic agar with added antibiotics for in-clinic AST. We investigated how well veterinary microbiologists and clinicians, without any prior experience, employ Flexicult Vet for the identification and AST of the most common canine and feline urinary pathogenic bacteria. We prepared 47 monoculture plates containing 10 bacterial species. The test’s mean accuracy was 75.1% for bacteria identification (84.6% and 68.7% for microbiologists and clinicians, respectively) and 79.2% for AST (80.7% and 78.2%). All evaluators employed Flexicult Vet with the accuracies over 90% for the distinctively colored bacteria like Escherichia coli (red), Enterococcus faecalis (turquoise), and Proteus spp. (pale brown). However, the evaluators’ experience proved important in recognizing lightly colored bacteria like Staphylococcus pseudintermedius (accuracies of 82.6% and 40.3%). Misidentifications of E. faecium additionally worsened AST performance since bacterial intrinsic resistance could not be considered. Finally, only 33.3% (3/9) of methicillin-resistant S. pseudintermedius (MRSP) were correctly detected. To conclude, Flexicult Vet proved reliable for certain urinary pathogens. In contrast, light-colored bacteria (e.g., Staphylococcus), often misidentified, require a standard AST

• Jezik: Angleški jezik
• Ključne besede: urinary tract infection, Flexicult Vet, antimicrobial susceptibility testing, pathogen identification, dogs, cats, veterinary microbiology
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo ; VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Datum objave: 24.09.2021
• Leto izida: 2021
• Št. strani: 10 str.
• Številčenje: Vol. 10, no. 10, art. 1160
• PID: 20.500.12556/RUL-132184
• UDK: 636.8.09:579:57.08
• ISSN pri članku: 2079-6382
• DOI: 10.3390/antibiotics10101160
• COBISS.SI-ID: 78002435
• Datum objave v RUL: 15.10.2021

Gradivo je del revije

• Naslov: Antibiotics
• Skrajšan naslov: Antibiotics
• Založnik: MDPI
• ISSN: 2079-6382
• COBISS.SI-ID: 522975769

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 24.09.2021

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Program financ.: Slovenian Ministry of Economic Development and Technology

• Financer: EC - European Commission
• Program financ.: European Regional Development Fund
• Številka projekta: Eureka E! 13509