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Analysis of the global population structure of Paenibacillus larvae and outbreak investigation of American foulbrood using a stable wgMLST scheme
Papić, Bojan (Avtor), Diricks, Margo (Avtor), Kušar, Darja (Avtor)

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URLURL - Izvorni URL, za dostop obiščite https://www.frontiersin.org/articles/10.3389/fvets.2021.582677/full Povezava se odpre v novem oknu

Izvleček
Paenibacillus larvae causes the American foulbrood (AFB), a highly contagious and devastating disease of honeybees. Whole-genome sequencing (WGS) has been increasingly used in bacterial pathogen typing, but rarely applied to study the epidemiology of P. larvae. To this end, we used 125 P. larvae genomes representative of a species-wide diversity to construct a stable whole-genome multilocus sequence typing (wgMLST) scheme consisting of 5745 loci. A total of 51 P. larvae isolates originating from AFB outbreaks in Slovenia were used to assess the epidemiological applicability of the developed wgMLST scheme. In addition, wgMLST was compared with the core-genome MLST (cgMLST) and whole-genome single nucleotide polymorphism (wgSNP) analyses. All three approaches successfully identified clusters of outbreak-associated strains, which were clearly separated from the epidemiologically unlinked isolates. High levels of backward comparability of WGS-based analyses with conventional typing methods (ERIC-PCR and MLST) were revealed; however, both conventional methods lacked sufficient discriminatory power to separate the outbreak clusters. The developed wgMLST scheme provides an improved understanding of the intra- and inter-outbreak genetic diversity of P. larvae and represents an important progress in unraveling the genomic epidemiology of this important honeybee pathogen.

Jezik:Angleški jezik
Ključne besede:Paenibacillus larvae, American foulbrood, whole-genome sequencing, whole-genome multilocus sequence typing, core-genome multilocus sequence typing, whole-genome single nucleotide polymorphism
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:VF - Veterinarska fakulteta
Leto izida:2021
Poslano v recenzijo:27.07.2020
Datum sprejetja članka:05.02.2021
Datum objave članka:26.02.2021
Št. strani:15 str.
Številčenje:Vol. 8, art. 582677
UDK:577
ISSN pri članku:2297-1769
DOI:10.3389/fvets.2021.582677 Povezava se odpre v novem oknu
COBISS.SI-ID:53310723 Povezava se odpre v novem oknu
Število ogledov:62
Število prenosov:25
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Gradivo je del revije

Naslov:Frontiers in veterinary science
Skrajšan naslov:Front. vet. sci.
Založnik:Frontiers Media
ISSN:2297-1769
COBISS.SI-ID:3969402 Povezava se odpre v novem oknu

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Program financ.:Ministrstvo za kmetijstvo, gozdarstvo in prehrano Republike Slovenije
Številka projekta:V4-1804
Naslov:Ugotavljanje poti širjenja hude gnilobe čebelje zalege z genetsko tipizacijo sevov povzročitelja bolezni

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:26.02.2021

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