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Genome-wide transcriptomic analysis reveals insights into the response to Citrus bark cracking viroid (CBCVd) in hop (Humulus lupulus L.)
ID Kumar Mishra, Ajay (Avtor), ID Kumar, Atul (Avtor), ID Mishra, Deepti (Avtor), ID Nath, Vishnu Sukumari (Avtor), ID Jakše, Jernej (Avtor), ID Kocábek, Tomáš (Avtor), ID Kumar Killi, Uday (Avtor), ID Morina, Filis (Avtor), ID Matoušek, Jaroslav (Avtor)

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Izvleček
Viroids are smallest known pathogen that consist of non-capsidated, single-stranded non-coding RNA replicons and they exploits host factors for their replication and propagation. The severe stunting disease caused by Citrus bark cracking viroid (CBCVd) is a serious threat, which spreads rapidly within hop gardens. In this study, we employed comprehensive transcriptome analyses to dissect host-viroid interactions and identify gene expression changes that are associated with disease development in hop. Our analysis revealed that CBCVd-infection resulted in the massive modulation of activity of over 2000 genes. Expression of genes associated with plant immune responses (protein kinase and mitogen-activated protein kinase), hypersensitive responses, phytohormone signaling pathways, photosynthesis, pigment metabolism, protein metabolism, sugar metabolism, and modification, and others were altered, which could be attributed to systemic symptom development upon CBCVd-infection in hop. In addition, genes encoding RNA-dependent RNA polymerase, pathogenesis-related protein, chitinase, as well as those related to basal defense responses were up-regulated. The expression levels of several genes identified from RNA sequencing analysis were confirmed by qRT-PCR. Our systematic comprehensive CBCVd-responsive transcriptome analysis provides a better understanding and insights into complex viroid-hop plant interaction. This information will assist further in the development of future measures for the prevention of CBCVd spread in hop fields.

Jezik:Angleški jezik
Ključne besede:Citrus bark cracking viroid, differentially expressed genes, hop, pathogen, transcriptome analysis, viroids
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2018
Št. strani:19 str.
Številčenje:Vol. 10, iss. 10, art. 570
PID:20.500.12556/RUL-132062 Povezava se odpre v novem oknu
UDK:577.2
ISSN pri članku:1999-4915
DOI:10.3390/v10100570 Povezava se odpre v novem oknu
COBISS.SI-ID:9078905 Povezava se odpre v novem oknu
Datum objave v RUL:11.10.2021
Število ogledov:1058
Število prenosov:143
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Viruses
Skrajšan naslov:Viruses
Založnik:MDPI
ISSN:1999-4915
COBISS.SI-ID:517597977 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:18.10.2018

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:genetske transformacije, viroid razpokanosti skorje agrumov, CBCVd, huda viroidna zakrnelost hmelja, Humulus lupulus, hmelj, transkriptomska analiza, različno izraženi geni

Projekti

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Operational Programme Research, Development and Education
Številka projekta:CZ.02.2.69/0.0/0.0/16_027/0008357
Akronim:MEMOBIC

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Czech Science foundation
Številka projekta:18-10515J/DFG STE 465/10-1

Financer:Drugi - Drug financer ali več financerjev
Številka projekta:CZ.02.1.01/0.0/0.0/15_003/0000336
Akronim:KOROLID

Financer:Drugi - Drug financer ali več financerjev
Številka projekta:RVO:60077344

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