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A novel strain of porcine adenovirus detected in urinary bladder urothelial cell culture
Dragin Jerman, Urška (Author), Kolenc, Marko (Author), Steyer, Andrej (Author), Veranič, Peter (Author), Poljšak-Prijatelj, Mateja (Author), Erdani-Kreft, Mateja (Author)

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Abstract
Contamination of cell cultures is the most common problem encountered in cell culture laboratories. Besides the secondary cell contaminations often occurring in the cell laboratories, the contaminations originating from donor animal or human tissue are equally as common, but usually harder to recognize and as such require special attention. The present study describes the detection of porcine adenovirus (PAdV), strain PAdV-SVN1 in cultures of normal porcine urothelial (NPU) cells isolated from urinary bladders of domestic pigs. NPU cell cultures were evaluated by light microscopy (LM), polymerase chain reaction (PCR), and additionally assessed by transmission electron microscopy (TEM). Characteristic ultrastructure of virions revealed the infection with adenovirus. The adenoviral contamination was further identified by the sequence analysis, which showed the highest similarity to recently described PAdV strain PAdV-WI. Additionally, the cell ultrastructural analysis confirmed the life-cycle characteristic for adenoviruses. To closely mimic the in vivo situation, the majority of research on in vitro models uses cell cultures isolated from human or animal tissue and their subsequent passages. Since the donor tissue could be a potential source of contamination, the microbiological screening of the excised tissue and harvested cell cultures is highly recommended.

Language:English
Keywords:porcine adenovirus, cell culture, urinary bladder, transmission electron microscopy, polymerase chain reaction, microbiological screening
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Year:2014
Number of pages:Str. 2505-2518
Numbering:Vol. 6, iss. 6
UDC:577
ISSN on article:1999-4915
DOI:10.3390/v6062505 This link opens in a new window
COBISS.SI-ID:31479257 This link opens in a new window
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Downloads:19
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Record is a part of a journal

Title:Viruses
Shortened title:Viruses
Publisher:MDPI
ISSN:1999-4915
COBISS.SI-ID:517597977 This link opens in a new window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P3-0108
Name:Diferenciacija urotelijskih celic

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P3-0083
Name:Odnosi parazitskega obstajanja

Licences

License:CC BY 3.0, Creative Commons Attribution 3.0 Unported
Link:https://creativecommons.org/licenses/by/3.0/deed.en
Description:You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Licensing start date:23.06.2014

Secondary language

Language:Slovenian
Keywords:prašičji adenovirus, celične kulture, sečni mehur

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