A novel strain of porcine adenovirus detected in urinary bladder urothelial cell culture

Dragin Jerman, Urška (author), Kolenc, Marko (author), Steyer, Andrej (author), Veranič, Peter (author), Poljšak-Prijatelj, Mateja (author), Erdani-Kreft, Mateja (author)

Contamination of cell cultures is the most common problem encountered in cell culture laboratories. Besides the secondary cell contaminations often occurring in the cell laboratories, the contaminations originating from donor animal or human tissue are equally as common, but usually harder to recognize and as such require special attention. The present study describes the detection of porcine adenovirus (PAdV), strain PAdV-SVN1 in cultures of normal porcine urothelial (NPU) cells isolated from urinary bladders of domestic pigs. NPU cell cultures were evaluated by light microscopy (LM), polymerase chain reaction (PCR), and additionally assessed by transmission electron microscopy (TEM). Characteristic ultrastructure of virions revealed the infection with adenovirus. The adenoviral contamination was further identified by the sequence analysis, which showed the highest similarity to recently described PAdV strain PAdV-WI. Additionally, the cell ultrastructural analysis confirmed the life-cycle characteristic for adenoviruses. To closely mimic the in vivo situation, the majority of research on in vitro models uses cell cultures isolated from human or animal tissue and their subsequent passages. Since the donor tissue could be a potential source of contamination, the microbiological screening of the excised tissue and harvested cell cultures is highly recommended.

• Language: English
• Keywords: porcine adenovirus, cell culture, urinary bladder, transmission electron microscopy, polymerase chain reaction, microbiological screening
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2014
• Number of pages: Str. 2505-2518
• Numbering: Vol. 6, iss. 6
• PID: 20.500.12556/RUL-130808
• UDC: 577
• ISSN on article: 1999-4915
• DOI: 10.3390/v6062505
• COBISS.SI-ID: 31479257
• Publication date in RUL: 17.09.2021

Record is a part of a journal

• Title: Viruses
• Shortened title: Viruses
• Publisher: MDPI
• ISSN: 1999-4915
• COBISS.SI-ID: 517597977

Licences

• License: CC BY 3.0, Creative Commons Attribution 3.0 Unported
• Link: https://creativecommons.org/licenses/by/3.0/deed.en
• Description: You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
• Licensing start date: 23.06.2014

Secondary language

• Language: Slovenian
• Keywords: prašičji adenovirus, celične kulture, sečni mehur

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0108
• Name: Diferenciacija urotelijskih celic

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0083
• Name: Odnosi parazitskega obstajanja