A novel strain of porcine adenovirus detected in urinary bladder urothelial cell culture

Dragin Jerman, Urška (avtor), Kolenc, Marko (avtor), Steyer, Andrej (avtor), Veranič, Peter (avtor), Poljšak-Prijatelj, Mateja (avtor), Erdani-Kreft, Mateja (avtor)

Contamination of cell cultures is the most common problem encountered in cell culture laboratories. Besides the secondary cell contaminations often occurring in the cell laboratories, the contaminations originating from donor animal or human tissue are equally as common, but usually harder to recognize and as such require special attention. The present study describes the detection of porcine adenovirus (PAdV), strain PAdV-SVN1 in cultures of normal porcine urothelial (NPU) cells isolated from urinary bladders of domestic pigs. NPU cell cultures were evaluated by light microscopy (LM), polymerase chain reaction (PCR), and additionally assessed by transmission electron microscopy (TEM). Characteristic ultrastructure of virions revealed the infection with adenovirus. The adenoviral contamination was further identified by the sequence analysis, which showed the highest similarity to recently described PAdV strain PAdV-WI. Additionally, the cell ultrastructural analysis confirmed the life-cycle characteristic for adenoviruses. To closely mimic the in vivo situation, the majority of research on in vitro models uses cell cultures isolated from human or animal tissue and their subsequent passages. Since the donor tissue could be a potential source of contamination, the microbiological screening of the excised tissue and harvested cell cultures is highly recommended.

• Jezik: Angleški jezik
• Ključne besede: porcine adenovirus, cell culture, urinary bladder, transmission electron microscopy, polymerase chain reaction, microbiological screening
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2014
• Št. strani: Str. 2505-2518
• Številčenje: Vol. 6, iss. 6
• PID: 20.500.12556/RUL-130808
• UDK: 577
• ISSN pri članku: 1999-4915
• DOI: 10.3390/v6062505
• COBISS.SI-ID: 31479257
• Datum objave v RUL: 17.09.2021

Gradivo je del revije

• Naslov: Viruses
• Skrajšan naslov: Viruses
• Založnik: MDPI
• ISSN: 1999-4915
• COBISS.SI-ID: 517597977

Licence

• Licenca: CC BY 3.0, Creative Commons Priznanje avtorstva 3.0 Nedoločena
• Povezava: https://creativecommons.org/licenses/by/3.0/deed.sl
• Opis: Dovoljuje kopiranje in razširjanje vsebin v kakršnemkoli mediju in obliki. Dovoljuje remixanje, urejanje, predelava in vključevanje vsebine v lastna dela v vse namene, tudi komercialne. Primerno morate navesti avtorja, povezavo do licence in označiti spremembe, če so kakšne nastale. To lahko storite na kakršenkoli razumen način, vendar ne na način, ki bi namigoval na to, da dajalec licence podpira vas ali vašo uporabo dela. Ne smete uporabiti pravnih določil ali tehničnih ukrepov, ki bi pravno omejili ali onemogočilo druge, da bi storili karkoli, kar licenca dovoli.
• Začetek licenciranja: 23.06.2014

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: prašičji adenovirus, celične kulture, sečni mehur

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0108
• Naslov: Diferenciacija urotelijskih celic

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0083
• Naslov: Odnosi parazitskega obstajanja