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Neurotoxicity of bupivacaine and liposome bupivacaine after sciatic nerve block in healthy and streptozotocin-induced diabetic mice
ID Markova, Liljana (Author), ID Umek, Nejc (Author), ID Horvat, Simon (Author), ID Hadžić, Admir (Author), ID Kuroda, Maxine M. (Author), ID Stopar Pintarič, Tatjana (Author), ID Mrak, Vesna (Author), ID Cvetko, Erika (Author)

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Abstract
Background: Long-acting local anaesthetics (e.g. bupivacaine hydrochloride) or sustained-release formulations of bupivacaine (e.g. liposomal bupivacaine) may be neurotoxic when applied in the setting of diabetic neuropathy. The aim of the study was to assess neurotoxicity of bupivacaine and liposome bupivacaine in streptozotocin (STZ) - induced diabetic mice after sciatic nerve block. We used the reduction in fibre density and decreased myelination assessed by G-ratio (defined as axon diameter divided by large fibre diameter) as indicators of local anaesthetic neurotoxicity. Results: Diabetic mice had higher plasma levels of glucose (P < 0.001) and significant differences in the tail flick and plantar test thermal latencies compared to healthy controls (P < 0.001). In both diabetic and nondiabetic mice, sciatic nerve block with 0.25% bupivacaine HCl resulted in a significantly greater G-ratio and an axon diameter compared to nerves treated with 1.3% liposome bupivacaine or saline (0.9% sodium chloride) (P < 0.01). Moreover, sciatic nerve block with 0.25% bupivacaine HCl resulted in lower fibre density and higher large fibre and axon diameters compared to the control (untreated) sciatic nerves in both STZ-induced diabetic (P < 0.05) and nondiabetic mice (P < 0.01). No evidence of acute or chronic inflammation was observed in any of the treatment groups. Conclusions: In our exploratory study the sciatic nerve block with bupivacaine HCl (7 mg/kg), but not liposome bupivacaine (35 mg/kg) or saline, resulted in histomorphometric indices of neurotoxicity. Histologic findings were similar in diabetic and healthy control mice.

Language:English
Keywords:bupivacaine hydrochloride, diabetes, neurotoxicity, liposome bupivacaine injectable suspension, peripheral neuropathy
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2020
Number of pages:8 str.
Numbering:Vol. 16, art. 247
PID:20.500.12556/RUL-128775 This link opens in a new window
UDC:615.2
ISSN on article:1746-6148
DOI:10.1186/s12917-020-02459-4 This link opens in a new window
COBISS.SI-ID:23004675 This link opens in a new window
Publication date in RUL:28.07.2021
Views:947
Downloads:185
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Record is a part of a journal

Title:BMC veterinary research
Publisher:BioMed Central.
ISSN:1746-6148
COBISS.SI-ID:515674393 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:17.07.2020

Secondary language

Language:Slovenian
Keywords:bupivakain hidroklorid, diabetes, nevrotoksičnost, injicirana suspenzija liposomskega bupivakaina, periferalna nevropatija

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P3-0043
Name:Molekularni mehanizmi razvoja in delovanja skeletne mišice

Funder:ARRS - Slovenian Research Agency
Project number:P4-0220
Name:Primerjalna genomika in genomska biodiverziteta

Funder:Other - Other funder or multiple funders
Funding programme:Clinical Department of Anaesthesiology and Intensive Therapy, University Clinical Centre (Ljubljana, Slovenia); tertiary funding

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