20.500.12556/RUL-128775 Neurotoxicity of bupivacaine and liposome bupivacaine after sciatic nerve block in healthy and streptozotocin-induced diabetic mice Background: Long-acting local anaesthetics (e.g. bupivacaine hydrochloride) or sustained-release formulations of bupivacaine (e.g. liposomal bupivacaine) may be neurotoxic when applied in the setting of diabetic neuropathy. The aim of the study was to assess neurotoxicity of bupivacaine and liposome bupivacaine in streptozotocin (STZ) - induced diabetic mice after sciatic nerve block. We used the reduction in fibre density and decreased myelination assessed by G-ratio (defined as axon diameter divided by large fibre diameter) as indicators of local anaesthetic neurotoxicity. Results: Diabetic mice had higher plasma levels of glucose (P < 0.001) and significant differences in the tail flick and plantar test thermal latencies compared to healthy controls (P < 0.001). In both diabetic and nondiabetic mice, sciatic nerve block with 0.25% bupivacaine HCl resulted in a significantly greater G-ratio and an axon diameter compared to nerves treated with 1.3% liposome bupivacaine or saline (0.9% sodium chloride) (P < 0.01). Moreover, sciatic nerve block with 0.25% bupivacaine HCl resulted in lower fibre density and higher large fibre and axon diameters compared to the control (untreated) sciatic nerves in both STZ-induced diabetic (P < 0.05) and nondiabetic mice (P < 0.01). No evidence of acute or chronic inflammation was observed in any of the treatment groups. Conclusions: In our exploratory study the sciatic nerve block with bupivacaine HCl (7 mg/kg), but not liposome bupivacaine (35 mg/kg) or saline, resulted in histomorphometric indices of neurotoxicity. Histologic findings were similar in diabetic and healthy control mice. bupivacaine hydrochloride diabetes neurotoxicity liposome bupivacaine injectable suspension peripheral neuropathy bupivakain hidroklorid diabetes nevrotoksičnost injicirana suspenzija liposomskega bupivakaina periferalna nevropatija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-28 13:57:36 2021-07-28 13:57:39 2022-09-07 04:17:08 0000-00-00 00:00:00 2020 0 0 8 str. Vol. 16, art. 247 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.2 1746-6148 10.1186/s12917-020-02459-4 23004675 RAZ_Markova_Liljana_2020.pdf RAZ_Markova_Liljana_2020.pdf 1 F87A1956C61AC39FDD73D6D18D152EFC 8968f380e8651fe2d7277b7048161b06791ff25357b770ed39733ba17073e371 d6b990a6-ef9a-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145646 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02459-4 0 efe180d9-ef9a-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145647 Medicinska fakulteta Biotehniška fakulteta 0 0 0