Neurotoxicity of bupivacaine and liposome bupivacaine after sciatic nerve block in healthy and streptozotocin-induced diabetic mice

Markova, Liljana (avtor), Umek, Nejc (avtor), Horvat, Simon (avtor), Hadžić, Admir (avtor), Kuroda, Maxine M. (avtor), Stopar Pintarič, Tatjana (avtor), Mrak, Vesna (avtor), Cvetko, Erika (avtor)

Background: Long-acting local anaesthetics (e.g. bupivacaine hydrochloride) or sustained-release formulations of bupivacaine (e.g. liposomal bupivacaine) may be neurotoxic when applied in the setting of diabetic neuropathy. The aim of the study was to assess neurotoxicity of bupivacaine and liposome bupivacaine in streptozotocin (STZ) - induced diabetic mice after sciatic nerve block. We used the reduction in fibre density and decreased myelination assessed by G-ratio (defined as axon diameter divided by large fibre diameter) as indicators of local anaesthetic neurotoxicity. Results: Diabetic mice had higher plasma levels of glucose (P < 0.001) and significant differences in the tail flick and plantar test thermal latencies compared to healthy controls (P < 0.001). In both diabetic and nondiabetic mice, sciatic nerve block with 0.25% bupivacaine HCl resulted in a significantly greater G-ratio and an axon diameter compared to nerves treated with 1.3% liposome bupivacaine or saline (0.9% sodium chloride) (P < 0.01). Moreover, sciatic nerve block with 0.25% bupivacaine HCl resulted in lower fibre density and higher large fibre and axon diameters compared to the control (untreated) sciatic nerves in both STZ-induced diabetic (P < 0.05) and nondiabetic mice (P < 0.01). No evidence of acute or chronic inflammation was observed in any of the treatment groups. Conclusions: In our exploratory study the sciatic nerve block with bupivacaine HCl (7 mg/kg), but not liposome bupivacaine (35 mg/kg) or saline, resulted in histomorphometric indices of neurotoxicity. Histologic findings were similar in diabetic and healthy control mice.

• Jezik: Angleški jezik
• Ključne besede: bupivacaine hydrochloride, diabetes, neurotoxicity, liposome bupivacaine injectable suspension, peripheral neuropathy
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 8 str.
• Številčenje: Vol. 16, art. 247
• PID: 20.500.12556/RUL-128775
• UDK: 615.2
• ISSN pri članku: 1746-6148
• DOI: 10.1186/s12917-020-02459-4
• COBISS.SI-ID: 23004675
• Datum objave v RUL: 28.07.2021

Gradivo je del revije

• Naslov: BMC veterinary research
• Založnik: Springer Nature
• ISSN: 1746-6148
• COBISS.SI-ID: 515674393

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 17.07.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: bupivakain hidroklorid, diabetes, nevrotoksičnost, injicirana suspenzija liposomskega bupivakaina, periferalna nevropatija