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Intrahost norovirus evolution in chronic infection over 5 years of shedding in a kidney transplant recipient
ID Steyer, Andrej (Avtor), ID Konte, Tilen (Avtor), ID Sagadin, Martin (Avtor), ID Kolenc, Marko (Avtor), ID Škoberne, Andrej (Avtor), ID Germ, Julija (Avtor), ID Dovč, Tadeja (Avtor), ID Arnol, Miha (Avtor), ID Poljšak-Prijatelj, Mateja (Avtor)

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Izvleček
Noroviruses are the leading cause of acute gastroenteritis, and they can affect humans of all age groups. In immunocompromised patients, norovirus infections can develop into chronic diarrhea or show prolonged asymptomatic virus shedding. Chronic norovirus infections are frequently reported for solid organ transplant recipients, with rapid intrahost norovirus evolution seen. In this report, we describe a case of chronic norovirus infection in an immunocompromised patient who was followed up for over 5 years. The purpose of the study was to specify the norovirus evolution in a chronically infected immunocompromised host and identify possible selection sites in norovirus capsid protein. During the follow-up period, 25 sequential stool samples were collected and nine of them were selected to generate amplicons covering viral RNA-dependent RNA polymerase (RdRp) and viral capsid protein (VP1) genes. Amplicons were sequenced using next-generation sequencing. Single nucleotide polymorphisms were defined, which demonstrated a nearly 3-fold greater mutation rate in the VP1 genome region compared to the RdRp genome region (7.9 vs. 2.8 variable sites/100 nucleotides, respectively). This indicates that mutations in the virus genome were not accumulated randomly, but are rather the result of mutant selection during the infection cycle. Using ShoRAH software we were able to reconstruct haplotypes occurring in each of the nine selected samples. The deduced amino-acid haplotype sequences were aligned and the positions were analyzed for selective pressure using the Datamonkey program. Only 12 out of 25 positive selection sites were within the commonly described epitopes A, B, C, and D of the VP1 protein. New positive selection sites were determined that have not been described before and might reflect adaptation of the norovirus toward optimal histo-blood-group antigen binding, or modification of the norovirus antigenic properties. These data provide new insights into norovirus evolutionary dynamics and indicate new putative epitope "hot-spots" of modified and optimized norovirus-host interactions.

Jezik:Angleški jezik
Ključne besede:norovirus, molecular evolution, chronic infection, solid organ transplantation, HBGA binding
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Leto izida:2018
Status objave pri reviji:Objavljeno
Verzija članka:Založnikova različica članka
Št. strani:12 str.
Številčenje:Vol. 9, art. 371
PID:20.500.12556/RUL-128737 Povezava se odpre v novem oknu
UDK:578
ISSN pri članku:1664-302X
DOI:10.3389/fmicb.2018.00371 Povezava se odpre v novem oknu
COBISS.SI-ID:33668057 Povezava se odpre v novem oknu
Datum objave v RUL:26.07.2021
Število ogledov:500
Število prenosov:118
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Frontiers in microbiology
Skrajšan naslov:Front. microbiol.
Založnik:Frontiers Research Foundation
ISSN:1664-302X
COBISS.SI-ID:4146296 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:02.03.2018

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:norovirus, molekularna evolucija, kronična okužba, presaditev organov, vezava HBGA

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P3-0083
Naslov:Odnosi parazitskega obstajanja

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