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Razvoj in matematični opis miniaturiziranega pretočnega sistema za ločevanje produktov biokatalitske transaminacije
ID Pilpah, Tilen (Author), ID Žnidaršič Plazl, Polona (Mentor) More about this mentor... This link opens in a new window

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Abstract
V nalogi sem izvajal separacijo acetofenona (ACP) in metilbenzilamina (MBA) v Y-oblikovanem mikrokanalu z vzporednim laminarnim tokom vodnega pufra z obema spojinama in n-heptana, v katerega se ekstrahira ACP. Modelna raztopina simulira reakcijski sistem transaminacije za proizvodnjo kiralnih aminov, katalizirane z ω-transaminazami. V eksperimentalnem delu sem izbral ustrezne obratovalne pogoje za učinkovito ekstrakcijo in ločbo faz na izstopu iz mikrokanala. Za opis sistema sem na osnovi matematičnega modela in posebej za to načrtovanih eksperimentov določil difuzivnostna koeficienta ACP in MBA v obeh fazah in njuna porazdelitvena koeficienta med fazama. V nadaljevanju sem razvil matematični model za opis ekstrakcije v vzporednem toku obeh kapljevin ter eksperimentalno izvedel ekstrakcijo v mikrokanalu pri različnih obratovalnih pogojih. Preko eksperimentalnih podatkov o vhodnih in izhodnih koncentracijah ACP in MBA sem prilagodil matematični model za opis danega sistema. Matematični model je dobro napovedal delovanje miniaturiziranega ekstraktorja, saj so se rezultati simulacij dobro skladali z eksperimentalno pridobljenimi vrednostmi. Mikrokanal pa se je izkazal za učinkovito napravo za ekstrakcijo, saj je dosegel preko 73 % izkoristek ekstrakcije pri zadrževalnem času manj kot 0,8 s.

Language:Slovenian
Keywords:ekstrakcija, separacija, kiralni amini, mikropretočne naprave, matematično modeliranje
Work type:Master's thesis/paper (mb22)
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Year:2021
COBISS.SI-ID:73955587 This link opens in a new window
Publication date in RUL:09.07.2021
Views:282
Downloads:50
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Secondary language

Language:English
Title:Development and mathematical description of a microflow system for biocatalytic transamination products separation
Abstract:
In the thesis, the separation of acetophenone (ACP) and methylbenzylamine (MBA) was performed in a Y-shaped microchannel with a parallel laminar flow of aqueous buffer containing both compounds and n-heptane into which ACP is extracted. The model solution simulates a transamination reaction system for the production of chiral amines catalyzed by ω-transaminases. In the experimental part, the appropriate operating conditions for efficient extraction and phase separation at the exit of the microchannel were selected. To describe the system, the diffusion coefficients of ACP and MBA in both phases and their distribution coefficients between phases were determined on the basis of a mathematical model and specially designed experiments. Next, a mathematical model to describe the extraction in parallel flow of both liquids was developed and the extraction in a microchannel was performed experimentally under different operating conditions. Through experimental data on ACP and MBA input and output concentrations, a mathematical model to describe a given system was adapted. The mathematical model predicted the operation of the miniaturized extractor well, as the simulation results were in good agreement with the experimentally obtained values. The microchannel proved to be an efficient extraction device, achieving over 73 % extraction efficiency at a retention time of less than 0.8 s.

Keywords:extraction, separation, chiral amines, microflow devices, mathematical modeling

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