20.500.12556/RUL-128373 Razvoj in matematični opis miniaturiziranega pretočnega sistema za ločevanje produktov biokatalitske transaminacije Development and mathematical description of a microflow system for biocatalytic transamination products separation V nalogi sem izvajal separacijo acetofenona (ACP) in metilbenzilamina (MBA) v Y-oblikovanem mikrokanalu z vzporednim laminarnim tokom vodnega pufra z obema spojinama in n-heptana, v katerega se ekstrahira ACP. Modelna raztopina simulira reakcijski sistem transaminacije za proizvodnjo kiralnih aminov, katalizirane z ω-transaminazami. V eksperimentalnem delu sem izbral ustrezne obratovalne pogoje za učinkovito ekstrakcijo in ločbo faz na izstopu iz mikrokanala. Za opis sistema sem na osnovi matematičnega modela in posebej za to načrtovanih eksperimentov določil difuzivnostna koeficienta ACP in MBA v obeh fazah in njuna porazdelitvena koeficienta med fazama. V nadaljevanju sem razvil matematični model za opis ekstrakcije v vzporednem toku obeh kapljevin ter eksperimentalno izvedel ekstrakcijo v mikrokanalu pri različnih obratovalnih pogojih. Preko eksperimentalnih podatkov o vhodnih in izhodnih koncentracijah ACP in MBA sem prilagodil matematični model za opis danega sistema. Matematični model je dobro napovedal delovanje miniaturiziranega ekstraktorja, saj so se rezultati simulacij dobro skladali z eksperimentalno pridobljenimi vrednostmi. Mikrokanal pa se je izkazal za učinkovito napravo za ekstrakcijo, saj je dosegel preko 73 % izkoristek ekstrakcije pri zadrževalnem času manj kot 0,8 s. In the thesis, the separation of acetophenone (ACP) and methylbenzylamine (MBA) was performed in a Y-shaped microchannel with a parallel laminar flow of aqueous buffer containing both compounds and n-heptane into which ACP is extracted. The model solution simulates a transamination reaction system for the production of chiral amines catalyzed by ω-transaminases. In the experimental part, the appropriate operating conditions for efficient extraction and phase separation at the exit of the microchannel were selected. To describe the system, the diffusion coefficients of ACP and MBA in both phases and their distribution coefficients between phases were determined on the basis of a mathematical model and specially designed experiments. Next, a mathematical model to describe the extraction in parallel flow of both liquids was developed and the extraction in a microchannel was performed experimentally under different operating conditions. Through experimental data on ACP and MBA input and output concentrations, a mathematical model to describe a given system was adapted. The mathematical model predicted the operation of the miniaturized extractor well, as the simulation results were in good agreement with the experimentally obtained values. The microchannel proved to be an efficient extraction device, achieving over 73 % extraction efficiency at a retention time of less than 0.8 s. ekstrakcija separacija kiralni amini mikropretočne naprave matematično modeliranje extraction separation chiral amines microflow devices mathematical modeling true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2021-07-09 17:10:00 2021-07-09 17:10:11 2022-09-07 04:14:46 0000-00-00 00:00:00 2021 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 7921 73955587 Pilpah_Tilen_-_Razvoj_in_matematicni_opis_miniaturiziranega_pretocnega_sistema_za_locevanje_prod.pdf Pilpah_Tilen_-_Razvoj_in_matematicni_opis_miniaturiziranega_pretocnega_sistema_za_locevanje_prod.pdf 1 118887E90EDCBEA77180C1CD19476C1C 7a8d32da1d1522d3af77f09a2221973a7c23bcb35d7e211108b151319f66b3f8 9e0d5bac-e0c7-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145097 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0