In the thesis, the separation of acetophenone (ACP) and methylbenzylamine (MBA) was performed in a Y-shaped microchannel with a parallel laminar flow of aqueous buffer containing both compounds and n-heptane into which ACP is extracted. The model solution simulates a transamination reaction system for the production of chiral amines catalyzed by ω-transaminases. In the experimental part, the appropriate operating conditions for efficient extraction and phase separation at the exit of the microchannel were selected. To describe the system, the diffusion coefficients of ACP and MBA in both phases and their distribution coefficients between phases were determined on the basis of a mathematical model and specially designed experiments. Next, a mathematical model to describe the extraction in parallel flow of both liquids was developed and the extraction in a microchannel was performed experimentally under different operating conditions. Through experimental data on ACP and MBA input and output concentrations, a mathematical model to describe a given system was adapted. The mathematical model predicted the operation of the miniaturized extractor well, as the simulation results were in good agreement with the experimentally obtained values. The microchannel proved to be an efficient extraction device, achieving over 73 % extraction efficiency at a retention time of less than 0.8 s.
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