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Mapping the gene expression spectrum of mediator subunits in response to viroid infection in plants
ID Nath, Vishnu Sukumari (Author), ID Shrestha, Ankita (Author), ID Awasthi, Praveen (Author), ID Kumar Mishra, Ajay (Author), ID Kocábek, Tomáš (Author), ID Matoušek, Jaroslav (Author), ID Sečnik, Andrej (Author), ID Jakše, Jernej (Author), ID Radišek, Sebastjan (Author), ID Hallan, Vipin (Author)

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Abstract
The mediator (MED) represents a large, conserved, multi-subunit protein complex that regulates gene expression through interactions with RNA polymerase II and enhancer-bound transcription factors. Expanding research accomplishments suggest the predominant role of plant MED subunits in the regulation of various physiological and developmental processes, including the biotic stress response against bacterial and fungal pathogens. However, the involvement of MED subunits in virus/viroid pathogenesis remains elusive. In this study, we investigated for the first time the gene expression modulation of selected MED subunits in response to five viroid species (Apple fruit crinkle viroid (AFCVd), Citrus bark cracking viroid (CBCVd), Hop latent viroid (HLVd), Hop stunt viroid (HSVd), and Potato spindle tuber viroid (PSTVd)) in two model plant species (Nicotiana tabacum and N. benthamiana) and a commercially important hop (Humulus lupulus) cultivar. Our results showed a differential expression pattern of MED subunits in response to a viroid infection. The individual plant MED subunits displayed a differential and tailored expression pattern in response to different viroid species, suggesting that the MED expression is viroid- and plant species-dependent. The explicit evidence obtained from our results warrants further investigation into the association of the MED subunit with symptom development. Together, we provide a comprehensive portrait of MED subunit expression in response to viroid infection and a plausible involvement of MED subunits in fine-tuning transcriptional reprogramming in response to viroid infection, suggesting them as a potential candidate for rewiring the defense response network in plants against pathogens.

Language:English
Keywords:differential expression, hop, mediator complex, Nicotiana benthamiana, Nicotiana tabacum, pathogen, quantitative reverse transcription PCR, viroid
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2020
Number of pages:10 str.
Numbering:Vol. 21, iss. 7, art. 2498
PID:20.500.12556/RUL-128258 This link opens in a new window
UDC:577.2
ISSN on article:1422-0067
DOI:10.3390/ijms21072498 This link opens in a new window
COBISS.SI-ID:9458041 This link opens in a new window
Publication date in RUL:07.07.2021
Views:765
Downloads:129
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Record is a part of a journal

Title:International journal of molecular sciences
Shortened title:Int. j. mol. sci.
Publisher:MDPI
ISSN:1422-0067
COBISS.SI-ID:2779162 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.04.2020

Secondary language

Language:Slovenian
Keywords:hmelj, genetika, izražanje genov, Nicotiana benthamiana, Nicotiana tabacum, patogeni, viroidi, kvantitativna genetika, kvantitativna reverzna transkripcija, PCR

Projects

Funder:EC - European Commission
Funding programme:Operational Programme Research, Development and Education
Project number:CZ.02.2.69/0.0/0.0/16_027/0008357
Acronym:MEMOBIC

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P4-0077
Name:Kmetijske rastline - genetika in sodobne tehnologije

Funder:Drugi - Drug financer ali več financerjev
Funding programme:Institute of Plant Molecular Biology
Project number:RVO:60077344

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