20.500.12556/RUL-128258 Mapping the gene expression spectrum of mediator subunits in response to viroid infection in plants The mediator (MED) represents a large, conserved, multi-subunit protein complex that regulates gene expression through interactions with RNA polymerase II and enhancer-bound transcription factors. Expanding research accomplishments suggest the predominant role of plant MED subunits in the regulation of various physiological and developmental processes, including the biotic stress response against bacterial and fungal pathogens. However, the involvement of MED subunits in virus/viroid pathogenesis remains elusive. In this study, we investigated for the first time the gene expression modulation of selected MED subunits in response to five viroid species (Apple fruit crinkle viroid (AFCVd), Citrus bark cracking viroid (CBCVd), Hop latent viroid (HLVd), Hop stunt viroid (HSVd), and Potato spindle tuber viroid (PSTVd)) in two model plant species (Nicotiana tabacum and N. benthamiana) and a commercially important hop (Humulus lupulus) cultivar. Our results showed a differential expression pattern of MED subunits in response to a viroid infection. The individual plant MED subunits displayed a differential and tailored expression pattern in response to different viroid species, suggesting that the MED expression is viroid- and plant species-dependent. The explicit evidence obtained from our results warrants further investigation into the association of the MED subunit with symptom development. Together, we provide a comprehensive portrait of MED subunit expression in response to viroid infection and a plausible involvement of MED subunits in fine-tuning transcriptional reprogramming in response to viroid infection, suggesting them as a potential candidate for rewiring the defense response network in plants against pathogens. differential expression hop mediator complex Nicotiana benthamiana Nicotiana tabacum pathogen quantitative reverse transcription PCR viroid hmelj genetika izražanje genov Nicotiana benthamiana Nicotiana tabacum patogeni viroidi kvantitativna genetika kvantitativna reverzna transkripcija PCR true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-07 14:09:44 2021-07-07 14:09:45 2022-09-07 04:14:01 0000-00-00 00:00:00 2020 0 0 10 str. iss. 7, art. 2498 Vol. 21 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 1422-0067 10.3390/ijms21072498 9458041 RAZ_Nath_Vishnu_Sukumari_2020.pdf RAZ_Nath_Vishnu_Sukumari_2020.pdf 1 5E5C41D25A74D0BF99AE20916D9DB78D ae77c3f9478d6946eb8c54d8d0483a3a416fc0f718ac292ef437493a24420a33 7873561d-df1c-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=144938 https://www.mdpi.com/1422-0067/21/7/2498 0 45f5a79c-df1c-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=144937 Biotehniška fakulteta 0 0 0