Darkening of the Greenland Ice Sheet : fungal abundance and diversity are associated with algal bloom

Perini, Laura (avtor), Gostinčar, Cene (avtor), Anesio, Alexandre (avtor), Williamson, Christopher (avtor), Tranter, Martyn (avtor), Gunde-Cimerman, Nina (avtor)

Recent studies have highlighted the importance of ice-algal blooms in driving darkening and therefore surface melt of the Greenland Ice Sheet (GrIS). However, the contribution of fungal and bacterial communities to this microbially driven albedo reduction remains unconstrained. To address this significant knowledge gap, fungi were isolated from key GrIS surface habitats (surface ice containing varying abundance of ice algae, supraglacial water, cryoconite holes, and snow), and a combination of cultivation and sequencing methods utilized to characterize the algal-associated fungal and bacterial diversity and abundance. Six hundred and ninety-seven taxa of fungi were obtained by amplicon sequencing and more than 200 fungal cultures belonging to 46 different species were isolated through cultivation approaches. Basidiomycota dominated in surface ice and water samples, and Ascomycota in snow samples. Amplicon sequencing revealed that bacteria were characterized by a higher diversity (883 taxa detected). Results from cultivation as well as ergosterol analyses suggested that surface ice dominated by ice algae and cryoconite holes supported the highest fungal biomass (10$^4$–10$^5$ CFU/100 ml) and that many fungal taxa recognized as endophytes and plant pathogens were associated with dark ice characterized by a high abundance of ice algae. This paper significantly advances this field of research by investigating for the first time the fungal abundance and diversity associated with algal blooms causing the darkening of the GrIS. There is a strong association between the abundance and diversity of fungal species and the blooming of algae on the surface ice of the Greenland Ice Sheet.

• Jezik: Angleški jezik
• Ključne besede: fungi, bacteria, Greenland Ice Sheet, dark ice, ice algae, NGS, microbial diversity, albedo effect
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2019
• Št. strani: 14 str.
• Številčenje: Vol. 10, art. 557
• PID: 20.500.12556/RUL-128253
• UDK: 579
• ISSN pri članku: 1664-302X
• DOI: 10.3389/fmicb.2019.00557
• COBISS.SI-ID: 5018447
• Datum objave v RUL: 07.07.2021

Gradivo je del revije

• Naslov: Frontiers in microbiology
• Skrajšan naslov: Front. microbiol.
• Založnik: Frontiers Media
• ISSN: 1664-302X
• COBISS.SI-ID: 4146296

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 21.03.2019