Separation and purification of biomacromolecules based on microfluidics

Vicente, Filipa A. (avtor), Plazl, Igor (avtor), Ventura, Sónia P. M. (avtor), Žnidaršič Plazl, Polona (avtor)

Separation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and reduced waste production as compared to conventional processing. This review highlights microfluidics-based separation and purification of proteins and nucleic acids with the focus on liquid–liquid extractions, particularly with biocompatible aqueous two-phase systems, which represent a cost-effective and green alternative. A variety of microflow set-ups are shown to enable sustainable and efficient isolation of target biomolecules both for preparative, as well as for analytical purposes.

• Jezik: Angleški jezik
• Ključne besede: microflow systems, biomacromolecules, aqueous two-phase systems, downstream processing
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: Str. 4391-4410
• Številčenje: Vol. 22, iss. 14
• PID: 20.500.12556/RUL-127087
• UDK: 66.02
• ISSN pri članku: 1463-9262
• DOI: 10.1039/c9gc04362d
• COBISS.SI-ID: 1538561987
• Datum objave v RUL: 17.05.2021

Gradivo je del revije

• Naslov: Green chemistry
• Skrajšan naslov: Green chem.
• Založnik: Royal Society of Chemistry
• ISSN: 1463-9262
• COBISS.SI-ID: 24129285

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 19.03.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikropretočni sistemi, biomakromolekule, dvofazni vodni sistemi, zaključni procesi