izpis_h1_title_alt

ERIC and WGS typing of Paenibacillus larvae in Slovenia : investigation of ERIC I outbreaks
ID Žugelj, Alenka (Avtor), ID Papić, Bojan (Avtor), ID Zdovc, Irena (Avtor), ID Zajc, Urška (Avtor), ID Golob, Majda (Avtor), ID Avberšek, Jana (Avtor), ID Kušar, Darja (Avtor)

.pdfPDF - Predstavitvena datoteka, prenos (3,50 MB)
MD5: 2080882958F5836609D8D8C8C43143D6
URLURL - Izvorni URL, za dostop obiščite https://www.mdpi.com/2075-4450/12/4/362 Povezava se odpre v novem oknu

Izvleček
Paenibacillus larvae is the causative agent of American foulbrood (AFB), a fatal disease of honeybee brood. Here, we obtained 506 P. larvae isolates originating from honey or brood samples and from different geographic regions of Slovenia in the period 2017–2019. In the first part of the study, we conducted ERIC-PCR typing to assess the frequency of ERIC types in Slovenia. Capillary electrophoresis was used for the analysis of ERIC patterns, revealing good separation efficiency and enabling easy lane-to-lane comparisons. ERIC II was the predominant type (70.2%), followed by ERIC I (29.8%); two slightly altered ERIC I banding patterns were observed but were not considered relevant for the discrimination of ERIC types. No evident spatiotemporal clustering of ERIC types was observed. To assess the clonality of the outbreak-related P. larvae ERIC I isolates, 59 isolates of this type underwent whole-genome sequencing (WGS). Whole-genome multilocus sequence typing (wgMLST) revealed seven ERIC I-ST2 outbreak clusters (≤35 allele differences) with the median intra-outbreak diversity ranging from 7 to 27 allele differences. In all seven clusters, the transmission of P. larvae outbreak clone within a 3-km radius (AFB zone) was observed, which could be explained by the activity of honeybees. In three clusters, the transmission of the outbreak clone between geographically distant apiaries was revealed, which could be explained by the activities of beekeepers such as migratory beekeeping and trading of bee colonies. The present findings reinforce the importance of beekeeping activities in the transmission of P. larvae. WGS should be used as a reference typing method for the detection of P. larvae transmission clusters.

Jezik:Angleški jezik
Ključne besede:Paenibacillus larvae, American foulbrood (AFB), ERIC-PCR, whole-genome sequencing (WGS), transmission, outbreak investigation, molecular sequence data, genotype
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:VF - Veterinarska fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2021
Št. strani:11 str.
Številčenje:Vol. 12, iss. 4, art. 362
PID:20.500.12556/RUL-126567 Povezava se odpre v novem oknu
UDK:577
ISSN pri članku:2075-4450
DOI:10.3390/insects12040362 Povezava se odpre v novem oknu
COBISS.SI-ID:60749571 Povezava se odpre v novem oknu
Datum objave v RUL:28.04.2021
Število ogledov:1793
Število prenosov:191
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
:
Kopiraj citat
Objavi na:Bookmark and Share

Gradivo je del revije

Naslov:Insects
Skrajšan naslov:Insects
Založnik:MDPI
ISSN:2075-4450
COBISS.SI-ID:519122457 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:19.04.2021

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:V4-1804
Naslov:Ugotavljanje poti širjenja hude gnilobe čebelje zalege z genetsko tipizacijo sevov povzročitelja bolezni

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0092
Naslov:Zdravje živali, okolje in varna hrana

Financer:Drugi - Drug financer ali več financerjev
Program financ.:Republic of Slovenia, Ministry of Agriculture, Forestry and Food

Podobna dela

Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:

Nazaj