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EpCAM homo-oligomerization is not the basis for its role in cell-cell adhesion
ID Gaber, Aljaž (Author), ID Kim, Seung Joong (Author), ID Kaake, Robyn M. (Author), ID Benčina, Mojca (Author), ID Krogan, Nevan J. (Author), ID Šali, Andrej (Author), ID Pavšič, Miha (Author), ID Lenarčič, Brigita (Author)

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Abstract
Cell-surface tumor marker EpCAM plays a key role in proliferation, diferentiation and adhesion processes in stem and epithelial cells. It is established as a cell-cell adhesion molecule, forming intercellular interactions through homophilic association. However, the mechanism by which such interactions arise has not yet been fully elucidated. Here, we frst show that EpCAM monomers do not associate into oligomers that would resemble an inter-cellular homo-oligomer, capable of mediating cell-cell adhesion, by using SAXS, XL-MS and bead aggregation assays. Second, we also show that EpCAM forms stable dimers on the surface of a cell with pre-formed cell-cell contacts using FLIM-FRET; however, no inter-cellular homo-oligomers were detectable. Thus, our study provides clear evidence that EpCAM indeed does not function as a homophilic cell adhesion molecule and therefore calls for a signifcantrevision of its role in both normal and cancerous tissues. In the light of this, we strongly support the previously suggested name Epithelial Cell Activating Molecule instead of the Epithelial Cell Adhesion Molecule.

Language:English
Keywords:epithelial cell adhesion molecule, cell adhesion, oligomerization
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Publication status:Published
Publication version:Version of Record
Year:2018
Number of pages:16 str.
Numbering:Vol. 8, art. 13269
PID:20.500.12556/RUL-125372 This link opens in a new window
UDC:577.112.85
ISSN on article:2045-2322
DOI:10.1038/s41598-018-31482-7 This link opens in a new window
COBISS.SI-ID:1537878979 This link opens in a new window
Publication date in RUL:12.03.2021
Views:686
Downloads:218
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Record is a part of a journal

Title:Scientific reports
Shortened title:Sci. rep.
Publisher:Springer Nature
ISSN:2045-2322
COBISS.SI-ID:18727432 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:12.03.2021

Secondary language

Language:Slovenian
Keywords:epitelijska celična adhezijska molekula, celična adhezija, oligomerizacija

Projects

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:J1-7119
Name:Biologija EpCAM na strukturnem nivoju kot osnova za učinkovito tumorsko ciljanje

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P1-0140
Name:Proteoliza in njena regulacija

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P1-0207
Name:Toksini in biomembrane

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