EpCAM homo-oligomerization is not the basis for its role in cell-cell adhesion

Gaber, Aljaž (avtor), Kim, Seung Joong (avtor), Kaake, Robyn M. (avtor), Benčina, Mojca (avtor), Krogan, Nevan J. (avtor), Šali, Andrej (avtor), Pavšič, Miha (avtor), Lenarčič, Brigita (avtor)

Cell-surface tumor marker EpCAM plays a key role in proliferation, diferentiation and adhesion processes in stem and epithelial cells. It is established as a cell-cell adhesion molecule, forming intercellular interactions through homophilic association. However, the mechanism by which such interactions arise has not yet been fully elucidated. Here, we frst show that EpCAM monomers do not associate into oligomers that would resemble an inter-cellular homo-oligomer, capable of mediating cell-cell adhesion, by using SAXS, XL-MS and bead aggregation assays. Second, we also show that EpCAM forms stable dimers on the surface of a cell with pre-formed cell-cell contacts using FLIM-FRET; however, no inter-cellular homo-oligomers were detectable. Thus, our study provides clear evidence that EpCAM indeed does not function as a homophilic cell adhesion molecule and therefore calls for a signifcantrevision of its role in both normal and cancerous tissues. In the light of this, we strongly support the previously suggested name Epithelial Cell Activating Molecule instead of the Epithelial Cell Adhesion Molecule.

• Jezik: Angleški jezik
• Ključne besede: epithelial cell adhesion molecule, cell adhesion, oligomerization
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2018
• Št. strani: 16 str.
• Številčenje: Vol. 8, art. 13269
• PID: 20.500.12556/RUL-125372
• UDK: 577.112.85
• ISSN pri članku: 2045-2322
• DOI: 10.1038/s41598-018-31482-7
• COBISS.SI-ID: 1537878979
• Datum objave v RUL: 12.03.2021

Gradivo je del revije

• Naslov: Scientific reports
• Skrajšan naslov: Sci. rep.
• Založnik: Nature Publishing Group
• ISSN: 2045-2322
• COBISS.SI-ID: 18727432

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 12.03.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: epitelijska celična adhezijska molekula, celična adhezija, oligomerizacija