20.500.12556/RUL-125372 EpCAM homo-oligomerization is not the basis for its role in cell-cell adhesion Cell-surface tumor marker EpCAM plays a key role in proliferation, diferentiation and adhesion processes in stem and epithelial cells. It is established as a cell-cell adhesion molecule, forming intercellular interactions through homophilic association. However, the mechanism by which such interactions arise has not yet been fully elucidated. Here, we frst show that EpCAM monomers do not associate into oligomers that would resemble an inter-cellular homo-oligomer, capable of mediating cell-cell adhesion, by using SAXS, XL-MS and bead aggregation assays. Second, we also show that EpCAM forms stable dimers on the surface of a cell with pre-formed cell-cell contacts using FLIM-FRET; however, no inter-cellular homo-oligomers were detectable. Thus, our study provides clear evidence that EpCAM indeed does not function as a homophilic cell adhesion molecule and therefore calls for a signifcantrevision of its role in both normal and cancerous tissues. In the light of this, we strongly support the previously suggested name Epithelial Cell Activating Molecule instead of the Epithelial Cell Adhesion Molecule. epithelial cell adhesion molecule cell adhesion oligomerization epitelijska celična adhezijska molekula celična adhezija oligomerizacija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-03-12 12:39:15 2021-03-12 12:39:15 2022-09-05 03:50:27 0000-00-00 00:00:00 2018 0 0 16 str. art. 13269 Vol. 8 2018 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.112.85 2045-2322 10.1038/s41598-018-31482-7 1537878979 18727432 RAZ_Gaber_Aljaz_2018.pdf RAZ_Gaber_Aljaz_2018.pdf 1 B2DDAD7DE7E76DEF696E680C52362327 628e70361e5172091fa7e4df9f2f8d2e054aba2f5b371731d3ae817c6fca6883 b2c8f20e-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141073 https://www.nature.com/articles/s41598-018-31482-7 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141072 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0