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Development of a microfluidic platform for R-phycoerythrin purification using an aqueous micellar two-phase system
Seručnik, Mojca (Avtor), Vicente, Filipa A. (Avtor), Brečko, Živa (Avtor), Coutinho, João A. P. (Avtor), Ventura, Sónia P. M. (Avtor), Žnidaršič Plazl, Polona (Avtor)

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URLURL - Izvorni URL, za dostop obiščite https://pubs.acs.org/doi/10.1021/acssuschemeng.0c05042 Povezava se odpre v novem oknu

Izvleček
Temperature-dependent aqueous micellar two-phase systems (AMTPSs) have recently been gaining attention in the isolation of high-added-value biomolecules from their natural sources. Despite their sustainability, aqueous two-phase systems, and particularly AMTPSs, have not been extensively applied in the industry, which might be changed by applying process integration and continuous manufacturing. Here, we report for the first time on an integrated microfluidic platform for fast and low-materialconsuming development of continuous protein purification using an AMTPS. A system comprised of a microchannel incubated at high temperature, enabling instantaneous triggering of a two-phase system formation, and a microsettler, allowing complete phase separation at the outlets, is reported here. The separation of phycobiliproteins and particularly the purification of R-phycoerythrin from the contaminant proteins present in the aqueous crude extract obtained from fresh cells of Gracilaria gracilis were thereby achieved. The results from the developed microfluidic system revealed that the fractionation performance was maintained while reducing the processing time more than 20-fold when compared with the conventional lab-scale batch process. Furthermore, the integration of a miniaturized ultrafiltration module resulted in the complete removal of the surfactant from the bottom phase containing R-phycoerythrin, as well as in nearly twofold target protein concentration. The process setup successfully exploits the benefits of process intensification along with the integration of various downstream processes. Further transfer to a meso-scale integrated system would make such a system appropriate for the separation and purification of biomolecules with high commercial interest.

Jezik:Angleški jezik
Ključne besede:purification, algae, microfluidics, ultrafiltration, protein purification, protein separation, process intensification, aqueous micellar two-phase systems
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Leto izida:2020
Št. strani:Str. 17097-17105
Številčenje:Vol. 8, iss. 46
UDK:66.081.63
ISSN pri članku:2168-0485
DOI:10.1021/acssuschemeng.0c05042 Povezava se odpre v novem oknu
COBISS.SI-ID:37812995 Povezava se odpre v novem oknu
Število ogledov:285
Število prenosov:288
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Gradivo je del revije

Naslov:ACS sustainable chemistry & engineering
Skrajšan naslov:ACS sustain. chem. & eng.
Založnik:American Chemical Society
ISSN:2168-0485
COBISS.SI-ID:519846169 Povezava se odpre v novem oknu

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P2-0191
Naslov:Kemijsko inženirstvo

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:N2-0067
Naslov:Večstopenjska sinteza z MIO-encimi v kontinuirnem mikroreaktorskem sistemu

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:J4-1775
Naslov:Razvoj mikropretočnih sistemov za analizo, izbor in uporabo bakterijskih celic

Financer:EC - European Commission
Program financ.:H2020
Številka projekta:811040
Naslov:Chair Of Micro Process Engineering and TEchnology
Akronim:COMPETE

Financer:Drugi - Drug financer ali več financerjev
Program financ.:CICECO-Aveiro Institute of Materials
Številka projekta:UIDB/50011/2020

Financer:Drugi - Drug financer ali več financerjev
Program financ.:CICECO-Aveiro Institute of Materials
Številka projekta:UIDP/50011/2020

Financer:Drugi - Drug financer ali več financerjev
Program financ.:FEDER
Številka projekta:PT2020 Partnership Agreement

Financer:Drugi - Drug financer ali več financerjev
Program financ.:FCT
Številka projekta:IF/00402/2015

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:17.12.2020

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:čiščenje, alge, mikrofluidika, ultrafiltracija, ločevanje proteinov, proteini, bioprocesi, vodni micelarni dvo-fazni sistemi

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