20.500.12556/RUL-122941 Development of a microfluidic platform for R-phycoerythrin purification using an aqueous micellar two-phase system Temperature-dependent aqueous micellar two-phase systems (AMTPSs) have recently been gaining attention in the isolation of high-added-value biomolecules from their natural sources. Despite their sustainability, aqueous two-phase systems, and particularly AMTPSs, have not been extensively applied in the industry, which might be changed by applying process integration and continuous manufacturing. Here, we report for the first time on an integrated microfluidic platform for fast and low-materialconsuming development of continuous protein purification using an AMTPS. A system comprised of a microchannel incubated at high temperature, enabling instantaneous triggering of a two-phase system formation, and a microsettler, allowing complete phase separation at the outlets, is reported here. The separation of phycobiliproteins and particularly the purification of R-phycoerythrin from the contaminant proteins present in the aqueous crude extract obtained from fresh cells of Gracilaria gracilis were thereby achieved. The results from the developed microfluidic system revealed that the fractionation performance was maintained while reducing the processing time more than 20-fold when compared with the conventional lab-scale batch process. Furthermore, the integration of a miniaturized ultrafiltration module resulted in the complete removal of the surfactant from the bottom phase containing R-phycoerythrin, as well as in nearly twofold target protein concentration. The process setup successfully exploits the benefits of process intensification along with the integration of various downstream processes. Further transfer to a meso-scale integrated system would make such a system appropriate for the separation and purification of biomolecules with high commercial interest. purification algae microfluidics ultrafiltration protein purification protein separation process intensification aqueous micellar two-phase systems čiščenje alge mikrofluidika ultrafiltracija ločevanje proteinov proteini bioprocesi vodni micelarni dvo-fazni sistemi true false true Angleški jezik Slovenski jezik Članek v reviji 2020-12-17 15:03:05 2020-12-17 15:03:06 2022-09-03 04:10:27 0000-00-00 00:00:00 2020 0 0 Str. 17097-17105 iss. 46 Vol. 8 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.081.63 2168-0485 10.1021/acssuschemeng.0c05042 37812995 519846169 RAZ_Serucnik_Mojca_2020.pdf RAZ_Serucnik_Mojca_2020.pdf 1 1CC8F990F2BB10B9CC46E2CE023E9CB1 817de91f3826af13fb21c63d8c4b3329e3898c3d70a3433cb1f2968b9be0253c 230bf556-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=138195 https://pubs.acs.org/doi/10.1021/acssuschemeng.0c05042 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=138193 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0