Introduction: Cytomegalovirus (CMV) belongs to the family of herpes viruses whose important feature is the latent settlement in the body and the possibility of subsequent reactivation, especially in the event of a decline in the immune system. Cytomegalovirus infection has no clear symptoms, manifests as influenza or mononucleosis, and rarely in more severe forms such as pneumonia or hemolytic anemia. Cytomegalovirus is transmitted by all body fluids (saliva, respiratory secretions, urine, sexual intercourse, blood, transfusion…), the incubation time is three to twelve weeks. An infected person can spread the virus for a long time after recovering from the infection. Globally, cytomegalovirus is the most common congenital infection, affecting 0.5 to 2.5% of live births. Education on the mode of transmission is currently the only option for preventing congenital cytomegalovirus infection. Purpose: The purpose of the thesis is to present cytomegalovirus, what are the consequences of infection with cytomegalovirus in pregnancy on the fetus and what are the long-term consequences. We are interested in how much midwifery students know about cytomegalovirus and how additional education through lectures affects knowledge of cytomegalovirus infection in pregnancy. Methods of work: The quantitative research method is used in the diploma thesis. We reviewed the available domestic and foreign literature on cytomegalovirus infection. At the same time, we conducted cytomegalovirus education among midwifery students. The effect of education on knowledge of cytomegalovirus infection was determined by a pre- and post-education survey. Basic descriptive measures and the Pearson Hi-square statistical test were used for analysis. Results: The results we obtained in the preparation of the diploma thesis indicate a lack of knowledge of midwifery students about cytomegalovirus. There is a visible difference before and after education to all participants in the research, but it seems a good idea to talk more about cytomegalovirus and to make a leaflet to raise awareness among pregnant women. We also used a statistical test to confirm our hypothesis that targeted education increases the awareness of midwifery students about this virus. Discussion and conclusion: Cytomegalovirus is a virus that we encounter often but know very little about. Healthcare professionals need targeted training to provide women with relevant information on preventing cytomegalovirus infection during pregnancy.