In the last few decades, the importance of versatile pyrazolidin-3-one derivatives has grown significantly. Important representatives of this group of organic molecules are bicyclic-3-pyrazolidinones, which are studied as potential antibiotics and antimalarials due to their high antimicrobial activity.
At the beginning of the study, a set of several crude pyrazolo[1,2-a]pyrazole derivatives were prepared and their potential inhibitory effect on the plasmodic enzyme dihydroorotate dehydrogenase was tested on the resulting reaction mixtures. The major and minor isomers were isolated from the reaction mixtures by medium pressure liquid chromatography (MPLC) and testing was repeated on those isomers that made up the mixture with increased activity.
In the second part of the study, we focused on the preparation of heterogeneous copper catalysts and examined their conversion efficiency on a selected [3+2] cycloaddition model reaction between azomethine imine and methyl propiolate. We were mainly interested in the mass of the catalyst and the reaction time required for the complete conversion of the reagents into a cycloaduct, as well as reconversion ability of already used catalyst. We wanted to generalize the model reaction to the largest possible chemical space by selecting differently substituted phenyl rings in the structures of azomethine imines.
We concluded our study with the synthesis of potential fluorescent markers of biological molecules activated by the N-hydroxysuccinimidyl and benzotriazolyl groups.
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