Chromatography is a laboratory technique where individual components of a mixture are separated according to their separation rate. The mixture is dissolved in the so-called mobile phase, which then travels through the stationary phase. The determination of the identity and concentration of molecules in the mixture is performed by analytical chromatography. Preparative chromatography, however, is used to prepare large quantities of pure substances of each molecular species that can be used later. Viruses and virus-like particles (molecules that resemble viruses but are non-infectious because they do not contain genetic material) and bacteriophages are nanoplexes and their processing depends on the complexity of the starting material, the number of purification methods to choose from, and the criteria which the end product has to match. Purified viral preparations have already been used as vaccines and have potential in gene therapy. Bacterial viruses or bacteriophages have already been proven to treat bacterial infections, but antibiotics have slowed researches regarding them. Due to the higher resistance of bacteria to antibiotics, they are again recognized as an alternative source of treatment for bacterial infections. It is for this reason that our study will examine the use of chromatography for virus isolation.
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