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Vitrification using soy lecithin and sucrose : a new way to store the sperm for the preservation of canine reproductive function
ID Zakošek, Maja (Avtor), ID Casal, Margret L. (Avtor), ID Šterbenc, Nataša (Avtor), ID Virant-Klun, Irma (Avtor), ID Mrkun, Janko (Avtor)

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Izvleček
A challenge in freezing semen for short and long-term availability is avoiding damage to intact spermatozoa caused by the freezing process. Vitrification protocols provide better results through less manipulation of semen and shorter freezing time compared to slow freezing techniques. Our research was aimed at improving vitrification methods for canine semen. Semen quality was determined in 20 ejaculates after collection. Each ejaculate was divided into eight aliquots, each with a dfferent extender. The control extender contained TRIS, citric acid, fructose, and antibiotics. Soy lecithin and sucrose were added to the control extender at different concentrations to make up the test extenders and final concentration of 50 X 10[sup]6 spermatozoa/mL. From each group, a 33 [micro]L (1.65 X 10[sup]6 spermatozoa) suspension of spermatozoa was dropped directly into liquid nitrogen and devitrified at least one week later and evaluated as before. Soy lecithin at 1% and 0.25 M sucrose added to the base vitrification media effectively preserved all sperm qualities. Our results demonstrate the effectiveness of our methods. Vitrification media containing sucrose and soy lecithin cause a minimal decline in quality of canine semen after devitrification. Furthermore, extenders used in our research did not contain egg yolk, which was replaced by soy lecithin, thus allowing for ease of shipping to other countries with strict requirements.

Jezik:Angleški jezik
Ključne besede:vitrification, soy lecithin, sucrose, semen preservation, dogs, way store the sperm, reproductive function, canine
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:VF - Veterinarska fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2020
Št. strani:15 str.
Številčenje:Vol. 10, iss. 4, art. 653
PID:20.500.12556/RUL-116660 Povezava se odpre v novem oknu
UDK:59
ISSN pri članku:2076-2615
DOI:10.3390/ani10040653 Povezava se odpre v novem oknu
COBISS.SI-ID:34795225 Povezava se odpre v novem oknu
Datum objave v RUL:01.06.2020
Število ogledov:1107
Število prenosov:292
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Animals
Skrajšan naslov:Animals
Založnik:MDPI AG
ISSN:2076-2615
COBISS.SI-ID:519120409 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:09.04.2020

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0053
Naslov:Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Naslov:American Slovenian bilateral project

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