The aim of the thesis is to determine the impact of large herbivore selective browsing on natural regeneration of tree and shrub species in the Karst region. Research plots included six deforested and partially enclosed areas created in 2012 in the vicinity of Divača. Analyses were conducted with pairwise comparison between fenced and unfenced areas. Vertical shielding of herb layers and all woody plants, for which we previously determined the type of species and height, were inventoried for each sample area (N = 48). Two years after the creation of research facilities we determined that differences between fenced and unfenced areas are present only in minimum rate and that large herbivore with browsing have most influence on natural regeneration of shrub species.