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Liposome destruction by hydrodynamic cavitation in comparison to chemical, physical and mechanical treatments
Pandur, Žiga (Avtor), Dogša, Iztok (Avtor), Dular, Matevž (Avtor), Stopar, David (Avtor)

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Izvleček
Liposomes are widely applied in research, diagnostics, medicine and in industry. In this study we show for the first time the effect of hydrodynamic cavitation on liposome stability and compare it to the effect of well described chemical, physical and mechanical treatments. Fluorescein loaded giant 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid vesicles were treated with hydrodynamic cavitation as promising method in inactivation of biological samples. Hydrodynamic treatment was compared to various chemical, physical and mechanical stressors such as ionic strength and osmolarity agents (glucose, Na+, Ca2+, and Fe3+), free radicals, shear stresses (pipetting, vortex mixing, rotational shear stress), high pressure, electroporation, centrifugation, surface active agents (Triton X-100, ethanol), microwave irradiation, heating, freezing-thawing, ultrasound (ultrasonic bath, sonotrode). The fluorescence intensity of individual fluorescein loaded lipid vesicles was measured with confocal laser microscopy. The distribution of lipid vesicle size, vesicle fluorescence intensity, and the number of fluorescein loaded vesicles was determined before and after treatment with different stressors. The different environmental stressors were ranked in order of their relative effect on liposome fluorescein release. Of all tested chemical, physical and mechanical treatments for stability of lipid vesicles, the most detrimental effect on vesicles stability had hydrodynamic cavitation, vortex mixing with glass beads and ultrasound. Here we showed, for the first time that hydrodynamic cavitation was among the most effective physico-chemical treatments in destroying lipid vesicles. This work provides a benchmark for lipid vesicle robustness to a variety of different physico-chemical and mechanical parameters important in lipid vesicle preparation and application.

Jezik:Angleški jezik
Ključne besede:hydrodynamic cavitation, giant lipid vesicles, DOPC, stability, fluorescein release, confocal laser microscopy
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FS - Fakulteta za strojništvo
Leto izida:2020
Št. strani:Str. 1-11
Številčenje:Vol. 61, art. 104826
UDK:577.11:576.3:547.915:620.193.16
ISSN pri članku:1350-4177
DOI:10.1016/j.ultsonch.2019.104826 Povezava se odpre v novem oknu
COBISS.SI-ID:5144696 Povezava se odpre v novem oknu
Število ogledov:346
Število prenosov:342
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Gradivo je del revije

Naslov:Ultrasonics Sonochemistry
Skrajšan naslov:Ultrason. sonochem.
Založnik:Elsevier
ISSN:1350-4177
COBISS.SI-ID:707668 Povezava se odpre v novem oknu

Gradivo je financirano iz projekta

Financer:EC - European Commission
Program financ.:H2020
Številka projekta:771567
Naslov:An investigation of the mechanisms at the interaction between cavitation bubbles and contaminants
Akronim:CABUM

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P2-0401
Naslov:Energetsko strojništvo

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P4-0116
Naslov:Mikrobiologija in biotehnologija živil in okolja

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:14.02.2020

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:modelne membrane, lipidni vezikli, hidrodinamska kavitacija, kemijski postopki, fizikalni postopki, mehanski postopki, fluorescein, fluorescenčna mikroskopija

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