20.500.12556/RUL-114099 Liposome destruction by hydrodynamic cavitation in comparison to chemical, physical and mechanical treatments Liposomes are widely applied in research, diagnostics, medicine and in industry. In this study we show for the first time the effect of hydrodynamic cavitation on liposome stability and compare it to the effect of well described chemical, physical and mechanical treatments. Fluorescein loaded giant 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid vesicles were treated with hydrodynamic cavitation as promising method in inactivation of biological samples. Hydrodynamic treatment was compared to various chemical, physical and mechanical stressors such as ionic strength and osmolarity agents (glucose, Na+, Ca2+, and Fe3+), free radicals, shear stresses (pipetting, vortex mixing, rotational shear stress), high pressure, electroporation, centrifugation, surface active agents (Triton X-100, ethanol), microwave irradiation, heating, freezing-thawing, ultrasound (ultrasonic bath, sonotrode). The fluorescence intensity of individual fluorescein loaded lipid vesicles was measured with confocal laser microscopy. The distribution of lipid vesicle size, vesicle fluorescence intensity, and the number of fluorescein loaded vesicles was determined before and after treatment with different stressors. The different environmental stressors were ranked in order of their relative effect on liposome fluorescein release. Of all tested chemical, physical and mechanical treatments for stability of lipid vesicles, the most detrimental effect on vesicles stability had hydrodynamic cavitation, vortex mixing with glass beads and ultrasound. Here we showed, for the first time that hydrodynamic cavitation was among the most effective physico-chemical treatments in destroying lipid vesicles. This work provides a benchmark for lipid vesicle robustness to a variety of different physico-chemical and mechanical parameters important in lipid vesicle preparation and application. hydrodynamic cavitation giant lipid vesicles DOPC stability fluorescein release confocal laser microscopy modelne membrane lipidni vezikli hidrodinamska kavitacija kemijski postopki fizikalni postopki mehanski postopki fluorescein fluorescenčna mikroskopija true false true Angleški jezik Slovenski jezik Članek v reviji 2020-02-14 13:28:04 2020-02-14 13:28:05 2022-08-26 03:49:06 0000-00-00 00:00:00 2020 0 0 11 str. art. 104826 Vol. 61 Mar. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.11:576.3:547.915:620.193.16 1350-4177 10.1016/j.ultsonch.2019.104826 5144696 RAZ_Pandur_Ziga_2020.pdf RAZ_Pandur_Ziga_2020.pdf 1 F3038631C2D5B6E74D94864CE34ACB49 18141b4a4c46947d91f1684b25d60eba99cd0b6fa958109f073223e2c891d40f 2e5cbe9b-a1b8-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=126224 RAZ_Pandur_Ziga_2020.docx RAZ_Pandur_Ziga_2020.docx 1 706653B15101CBE3BC7A8416E7D57FC9 d5ae3792a1b38b29e68759e434c3f1b8a98f517aa94ccf238c8626ddbb2f3ab9 e3080315-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=142229 https://www.sciencedirect.com/science/article/pii/S1350417719309228 0 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=126223 Biotehniška fakulteta Fakulteta za strojništvo 0 0 0