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Evaluation of human milk microbiota by 16S rRNA gene next-generation sequencing (NGS) and cultivation/MALDI-TOF mass spectrometry identification
ID Treven, Primož (Avtor), ID Mahnič, Aleksander (Avtor), ID Rupnik, Maja (Avtor), ID Golob, Majda (Avtor), ID Pirš, Tina (Avtor), ID Bogovič Matijašić, Bojana (Avtor), ID Mohar Lorbeg, Petra (Avtor)

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Opis: Corrigendum
URLURL - Izvorni URL, za dostop obiščite https://www.frontiersin.org/articles/10.3389/fmicb.2019.02612/full Povezava se odpre v novem oknu
To gradivo ima še več datotek. Celoten seznam je na voljo spodaj.

Izvleček
The aim of the present study was to characterize human milk microbiota (HMM) with 16S rRNA gene amplicon next-generation sequencing and cultivation/matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF) mass spectrometry (MS) identification approaches. We analyzed 31 human milk samples from healthy Slovenian mothers. To check the accuracy of MALDI-TOF MS identification, several colonies representing most abundant genera and those, which could not be reliably identified by MALDITOF, were subjected to Sanger sequencing of their 16S rRNA gene. We showed that cultivation/MALDI-TOF MS was a suitable tool for culture-dependent determination of HMM. With both approaches, Staphylococcus and Streptococcus were found as predominant genera in HMM and the abundance of Staphylococcus was associated with decreased microbial diversity. In addition, we characterized factors that might influence HMM. The use of a breast pump was significantly associated with composition of HMM, higher microbial load, and lower abundance of cultivable staphylococci. Moreover, our study suggests that administration of probiotics to the suckling infant might influence HMM by increased abundance of lactobacilli and the presence of viable probiotic bacteria in human milk. However, since our study was observational with relatively small sample size, more targeted studies are needed to study possible transfer of probiotics to the mammary gland via an external route and the physiological relevance of these events.

Jezik:Angleški jezik
Ključne besede:milk microbiota, microbiota assembly, breastfeeding, breast pump, probiotic drops
Vrsta gradiva:Članek v reviji
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
VF - Veterinarska fakulteta
Status publikacije:Objavljeno
Različica publikacije:Objavljena publikacija
Leto izida:2019
Št. strani:12 str.
Številčenje:Vol. 10, November, art. 2612
PID:20.500.12556/RUL-113787 Povezava se odpre v novem oknu
UDK:579
ISSN pri članku:1664-302X
DOI:10.3389/fmicb.2019.02612 Povezava se odpre v novem oknu
COBISS.SI-ID:4326792 Povezava se odpre v novem oknu
Datum objave v RUL:04.02.2020
Število ogledov:1606
Število prenosov:914
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
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Gradivo je del revije

Naslov:Frontiers in microbiology
Skrajšan naslov:Front. microbiol.
Založnik:Frontiers Research Foundation
ISSN:1664-302X
COBISS.SI-ID:4146296 Povezava se odpre v novem oknu

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:15.11.2019

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:mikrobiologija, humano mleko, mikrobiota, genetika, masna spektrometrija

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:Z3-8198
Naslov:Karakterizacija in vitro modela za študij mikrobiote mlečne žleze (akronim - MAGIC)

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P4-0097
Naslov:Prehrana in mikrobna ekologija prebavil

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