Protection of agricultural land is important from the perspective of ensuring sufficient food self-supply and on the other hand for protecting the most productive agricultural land, because they are irreplaceable. The protection of agricultural land is also linked to protection of the cultural landscape. Since in Slovenia we did not have a system for the protection of agricultural land in the past, we have lost 70.000 hectares of agricultural land since the independence in 1991 (Kako prenesti ..., 2017). The Government of the Republic of Slovenia has prepared the regulation for defining areas, which are strategically important for our country, while the Rules for specification of the proposal pertaining protected agricultural land (PPAL) provide detailed instructions for the designation of these areas. On the basis of rules and by using the ArcGIS program, we graded areas according to criteria and defined the permanently protected agricultural land (TVKZ), other agricultural land (OKZ) and areas, which are potentially suitable for agriculture (PTVKZ) in Municipality of Novo mesto. In order to determine the impact of certain criteria form rules, we have created different scenarios that exclude certain criteria. The result of the master thesis is a map of TVKZ, OKZ and PTVKZ areas, which is the proposal for protection of agricultural land and calculated areas of TVKZ, OKZ and PTVKZ in hectares.