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Characterization of antimicrobial resistance in lactobacilli and bifidobacteria used as probiotics or starter cultures based on integration of phenotypic and in silico data
ID
Rozman, Vita
(
Avtor
),
ID
Mohar Lorbeg, Petra
(
Avtor
),
ID
Accetto, Tomaž
(
Avtor
),
ID
Bogovič Matijašić, Bojana
(
Avtor
)
PDF - Predstavitvena datoteka,
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MD5: 4A472E28F530BAF0771713EDC570B39D
URL - Izvorni URL, za dostop obiščite
https://www.sciencedirect.com/science/article/pii/S0168160519303198
Galerija slik
Izvleček
Lactic acid bacteria and bifidobacteria deliberately introduced into the food chain may act as a reservoir of antimicrobial resistance genes (ARGs), which is considered a safety concern. In the present study, resistance to antimicrobials of commercial probiotic strains, probiotic candidate strains, and starter cultures (n = 20) was characterised based on integration of phenotypic and in silico data. Minimum inhibitory concentrations (MICs) of 16 antimicrobials were determined for lactobacilli and bifidobacteria that were isolated from pharmaceutical products or obtained from the manufacturers or culture collections. Using different databases and bioinformatic tools, we predicted ARGs, mutations, genomic islands, and mobile genetic elements (MGEs) in their whole genome sequences. In addition, a comprehensive in silico analysis of the prevalence of the tetW gene and its genetic environment across lactobacilli and bifidobacteria (n = 1423) was conducted. Several strains exhibited phenotypic resistance to kanamycin, tetracycline, chloramphenicol, quinupristin-dalfopristin, ciprofloxacin, or neomycin. These resistances, however, did not always correspond to the presence of ARGs and vice versa. We detected an acquired tetW gene in four commercial strains of Bifidobacterium animalis subsp. lactis, whereas homologs of antimicrobial resistance (AR) proteins were predicted in all 20 proteomes. The prevalence of the tetW gene, which was often flanked by MGEs, was higher in analysed bifidobacteria (31.9%) than lactobacilli (6.3%). In addition, sequences flanking tetW were associated with putative genomic islands and were conserved in several strains, including potential pathogens. Our findings provide an insight into AR of probiotics, probiotic candidates, and starter cultures with an emphasis on tetracycline and into the safety of these strains in the context of AR.
Jezik:
Angleški jezik
Ključne besede:
antimicrobial resistance
,
Bifidobacterium
,
genomic island
,
Lactobacillus
,
mobile genetic element
,
tetracycline
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2020
Št. strani:
11 str.
Številčenje:
Vol. 314, art. 108388
PID:
20.500.12556/RUL-113246
UDK:
579
ISSN pri članku:
1879-3460
DOI:
10.1016/j.ijfoodmicro.2019.108388
COBISS.SI-ID:
4320904
Datum objave v RUL:
30.01.2020
Število ogledov:
1380
Število prenosov:
613
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
International journal of food microbiology
Skrajšan naslov:
Int. j. food microbiol.
Založnik:
Elsevier
ISSN:
1879-3460
COBISS.SI-ID:
23238661
Licence
Licenca:
CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:
Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
mikrobiologija
,
laktobacili
,
bifidobakterije
,
probiotiki
,
odpornost
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
6316-1/2017-273
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
603-1/2017-13
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
J4-1769
Naslov:
Rezistomi probiotičnih in starterskih kultur kot potencialni dejavnik tveganja za širjenje odpornosti proti antibiotikom
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P4-0097
Naslov:
Prehrana in mikrobna ekologija prebavil
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