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Potential applications of rapid microbiological methods for detection of antibiotic residues in wastewater, surface and well water
Godič Torkar, Karmen (Avtor), Fink, Rok (Avtor)

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Izvleček
The goal of the present study was to determine whether the commercial microbiological and tracer assays for the detection of antibiotics in the food were also useful and sensitive enough for testing water samples. Diffusion tests Delvotest® SP-NT and BRT-AiM showed the similar sensitivity to tested antibiotics in spiked water samples. Both tests showed the similar sensitivity to examined antibiotics in water as it was published in milk, while tracer assay BetaStar showed slightly higher minimum detection levels for penicillin and ampicillin but not for cloxacillin. The previous concentration of the samples by lyophilization took place to detect concentrations of antibiotics 100-fold lower than there were the minimum detection limits of the assays. The presence of inhibitory substances in surface and well samples was detected in 16 (16.3 %) cases out of 98 with both ampoule diffusion methods. The positive results were obtained at 15.0 % of surface water samples, while in well water the residues were found also in 16.9 % and 13.6 % samples, using Delvotest SPNT and BRT-AiM, respectively. The β-lactams were detected with BetaStar in 7.5 % of surface water samples. The 12 wastewater samples from hospitals were contaminated with inhibitory substances in 45.5 % (Delvotest SP-NT) or in 36.4 % (BRT-AiM).

Jezik:Angleški jezik
Ključne besede:antibiotics, microbiological methods, water, screening, contamination
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:ZF - Zdravstvena fakulteta
Leto izida:2013
Št. strani:str. 31-47
Številčenje:Vol. 7, no. 1
UDK:614.777:579.68
ISSN pri članku:1854-0678
URN:URN:NBN:SI:DOC-QEXQHCJE
COBISS.SI-ID:4640875 Povezava se odpre v novem oknu
Licenca:CC BY-NC-ND 4.0
To delo je dosegljivo pod licenco Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Število ogledov:303
Število prenosov:152
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Naslov:Sanitarno inženirstvo
Založnik:Inštitut za sanitarno inženirstvo
ISSN:1854-0678
COBISS.SI-ID:216969216 Povezava se odpre v novem oknu

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Jezik:Slovenski jezik
Ključne besede:antibiotiki, mikrobiološke metode, voda, presejanje, onesnaženje

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