Introduction: Health-related infections are diseases or pathological changes that occur during the patient’s medical care. The most important part of them are hospital infections, as the usually affect the most vulnerable groups of patients. In teh period after 1980, the number of hospital infections began to increase exponentially. Pathogenic organisms are transmitted to infected or colonized patients on their skin, clothing, bedding and nearby surroundings. Most often, the entire patient’s environment is contaminated. Hand hygiene, cleaning and disinfection help to control the spread of pathogens in health care institutions. Purpose: In the first part of the study, we determined how individual health care institutions are responsible for controlling the cleaning and prevention of health-related infections at the stage of cleaning. In the second part, we experimentally tested the effectiveness of the cleaner used in the actual cleaning of the soil in one of the health institutions. We used the Staphylococcus aureus bacterium. In addition, we also checked the effectiveness of water flow to remove bacteria from the surface. Methods: The integration of two quantitative methods was used. The purpose of integration is complementarity, in which the results of one method served to deepen and expand the findings. A sequential research plan was used, in which the first phase was the collection of data with the help of a questionnaire, and the research was deepened with experimental work. The chosen research strategy was a cross-sectional study. Results: Based on the survey questionnaire, we found that in the healthcare institutions across Slovenia, there is a different procedure for cleaning and controling the cleaning. In the experimental part, by means of manual purification of bacteria from the surface of the soil, we came to the conclusion that the mechanical force of the bacterial arm was partially removed. Based on the work with the flow chamber, we confirm that the volume flow affects the removal of bacteria from the soil surface. Discussion and conclusion: With the results obtained, we partially confirmed that the institutions throughout Slovenia follow the Rules on Prevention and Control of Infections in Health Facilities and implement the program of prevention of hospital infections and take care of the controls. The experimental work confirmed the other three hypotheses that the purification efficiency depends mainly on the mechanical force or the number of sweeps in the surface and the use of the cleaning agent, and that the water flow statistically reduces the number of bacteria on the surface.
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