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Salmonella Infantis in broiler flocks in Slovenia : the prevalence of multidrug resistant strains with high genetic homogeneity and low biofilm-forming ability
Pate, Mateja (Avtor), Mićunović, Jasna (Avtor), Golob, Majda (Avtor), Vestby, Lene Karine (Avtor), Ocepek, Matjaž (Avtor)

URLURL - Izvorni URL, za dostop obiščite https://www.hindawi.com/journals/bmri/2019/4981463/ Povezava se odpre v novem oknu

Izvleček
For almost a decade, the number of Salmonella enterica subspecies enterica serovar Infantis-positive broiler flocks has been steadily increasing in Slovenia, doubling the number of positive holdings in only a few years. Since multidrug resistant S. Infantis isolates are highly prevalent in the broiler meat industry and may represent a public health concern through the food chain, we aimed to investigate the antimicrobial susceptibility, genetic diversity, and biofilm-forming ability of S. Infantis from Slovenian broiler flocks. A total of 87 S. Infantis strains isolated from broiler faeces in the period between 2007 and 2013 were studied. The samples originated from 41 farms which were subcontractors of three major food business operators and from two autonomously operating holdings (farms). Isolates were phenotypically tested for their susceptibility to 14 antimicrobials from nine classes by determining the minimum inhibitory concentration with the microdilution method. Only 8% of the isolates were susceptible to all of the antimicrobial agents tested, while 88.5% of the isolates were multidrug resistant, with the most common resistance pattern CipNxSSuT (65.5%) followed by CipNxSuT (17.2%). Pulsed-field gel electrophoresis (PFGE) divided the strains into five clusters (A-E) comprising 16 distinct XbaI PFGE profiles. Sixty-five out of 87 isolates were grouped in clusters A and B, with the predominant PFGE profiles A1 and B1 encompassing 33 and 28 isolates, respectively. A vast majority of the isolates (75/87) showed >90% PFGE profile similarity. The biofilm-forming capacity of the tested isolates, determined with crystal violet assay in polystyrene microwell plates, was generally weak. The average biofilm formation for persistent strains was higher than for presumably nonpersistent strains; however, the difference was not significant. It seems that S. Infantis persistence on broiler farms is more related to its widespread occurrence in the broiler production chain and ineffective disinfection protocols than to its ability to form biofilm.

Jezik:Angleški jezik
Ključne besede:Poultry diseases-drug therapy, Salmonella infections, animal-microbiology, biofilms-drug effects, chickens-microbiology, drug resistance, multiple, bacterial, feces-microbiology, genetic variation, microbial sensitivity tests, serogroup, Slovenia
Vrsta gradiva:Članek v reviji (dk_c)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:VF - Veterinarska fakulteta
Leto izida:2019
Založnik:Hindawi
Št. strani:Art. ID 4981463, str. 1-13
Številčenje:Vol. 2019
UDK:636.09:579.62:614
ISSN pri članku:2314-6141
DOI:10.1155/2019/4981463 Povezava se odpre v novem oknu
COBISS.SI-ID:4756602 Povezava se odpre v novem oknu
Število ogledov:532
Število prenosov:163
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Gradivo je del revije

Naslov:BioMed research international
Skrajšan naslov:Biomed Res Int
Založnik:Hindawi Pub. Co.
ISSN:2314-6141
COBISS.SI-ID:30624729 Povezava se odpre v novem oknu

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P4-0092
Naslov:Zdravje živali, okolje in varna hrana

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:23.07.2019

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