Among Slovenian people Gorenjski carnation holds a special place. Beside as a form of decorational plant we can find it at many special and important events. One of the options of reproduction and growing is by using explants. But with this method we can transfer some of many fungal diseases. To avoid fungal diseases nowadays we are more and more often using a method of plant tissue culture, more specific micropropragation. Micropropragation is a method of ''in vitro'' cultivation where in short period of time we can produce a mass amount of plants. The best results of the cultivation of carnation yielded with leaf explants in comparison with nodal and internodal. Those explants were transferred in MS (Murashige and Skoog) medium where they observedthe level and speed of development of the callus. This also depends on concentration of auxin and cytokinin in given medium. The test results show that 3,0 mg/l 2,4-D (2,4- dichlorophenoxyacetic acid) is the best amount of avksin in MS medium for the development of the callus. However the combination of 2,0 mg/l NAA (?- anphthalene acetic acid) with 1,0 mg/l BAP (6- benzylamino purine) is the best amount of auxin and cytokinin for the development of the callus. The callus also responded with excellent development in MS medium with the concentration of 4,0 mg/l BAP with combination of kinetin. It showed that the best incubation period for the development of the callus is 8 weeks at the optimum temperature of 27°C. As conclusion for the reproduction of the calluses the best results were achieved with MS medium with the additive of 1,0 mg/ NAA or 1,0 mg/ NAA together with 3,0 mg/l BAP. After wast amount of research it was shown that the problems can also develop with the said ''in vitro'' method. With the carnation it mostly results in vitrification.