The awareness of eating healthy food is rising, because of that, there is more studies about influences of different substances in fruits and vegetables on health. The emphasis is on phenolics. They are reducing the chance of some cardiovascular disease and cancer. Because the strawberries are very popular at consumers and they contain a lot of phenolics, we decided that we will compare three red fruited cultivars ('Clery', 'Albion' and 'Eliany') and two white fruited cultivars ('Natural albino' and 'Snow white'). We measured mass, hardness, and colour parameters and analyzed sugars, organic acids, vitamin C and phenolic compounds. The highest fruit mass had cultivar 'Albion' and the lowest 'Natural albino'. The softest fruits had cultivars 'Natural albino' and 'Snow white'. They were three to four times softer than the 'Albion' which had the most firm fruits. The results showed that there is no difference in content of total phenolic contents. Cultivar 'Natural albino' had the highest content of total sugars. There were no differences between cultivars in total organic acids. The highest content of vitamin C had fruits of 'Natural albino' (for 24% more than 'Eliany'), while 'Eliany' had the lowest. White cultivars had highest content of flavanones ('Natural albino') and derivates of hidroxycinnamic acids ('Snow white'). Cultivar 'Eliany' had higher content of hydroxybenzoic derivatives and flavanols. The highest content of flavones had cultivar 'Clery'. Flavonols content was the highest in cultivars 'Clery' and 'Albion'.