A pure tire pyrolysis oil produced from waste tires was utilized in a modern 4-cylinder, turbocharged and intercooled, automotive Diesel engine. Due to its low cetane number, cetane improvers, external energy addition or increased compression ratios are generally required for its use in Diesel engines. Successful utilization of pure tire pyrolysis oil is also achievable with the addition of pilot injection but limited to mid- to high-load operating range. The first objective of the present study is therefore focused on further extension of the operating range towards lower loads by novel combined application of the exhaust gas recirculation and tailored main injection strategy. As the second objective, the article provides for the first time an in-depth analysis of the particulate emissions of the tire pyrolysis oil measured with two different methods. In this area it identifies and reasons challenges related to determination of the particulate emissions for alternative fuels. The original contribution of the presented approach thus arises from the holistic assessment of interactions between the exhaust gas recirculation ratios, injection parameters and combustion as well as gaseous and particulate emissions formation phenomena.