Members of genus Streptomyces are know producers of various natural products, including different tetracycline antibiotics such as oxytetracycline. Tetracyclines are broad-spectrum antibiotics. Non-soluble complex media are used for industrial production of oxytetracycline; often causeing signifficant difficulties in analysis of fermentation process and other culture parameters.. It was therefore goal of this research development of soluble and chemically defined medium for biosynthesis of oxytetracycline. We replaced non-soluble components of complex medium with soluble alternaives. We replaced CaCO3 with CaCl2 with addition of buffer MOPS or MES. As soluble source of carbon we used dextrin and as soluble soruce of nitrogen we used ammonium sulphate. CSL has been removed from medium and mix of minerals was added. Athough much lower yiled of oxytetracycline was achieved, we stilč manage to retain sifficient yield by replacing different medium components in both, vegetative and productive media.
|
