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Production of extracellular heterologous proteins in Streptomyces rimosus, producer of the antibiotic oxytetracycline
Carrillo Rincon, Andres Felipe (Author), Magdevska, Vasilka (Author), Kranjc, Luka (Author), Fujs, Štefan (Author), Müller, Rolf (Author), Petković, Hrvoje (Author)

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Abstract
Among the Streptomyces species, Streptomyces lividans has often been used for the production of heterologous proteins as it can secrete target proteins directly into the culture medium. Streptomyces rimosus, on the other hand, has for long been used at an industrial scale for oxytetracycline production, and it holds ‘Generally Recognised As Safe’ status. There are a number of properties of S. rimosus that make this industrial strain an attractive candidate as a host for heterologous protein production, including (1) rapid growth rate; (2) growth as short fragments, as for Escherichia coli; (3) high efficiency of transformation by electroporation; and (4) secretion of proteins into the culture medium. In this study, we specifically focused our efforts on an exploration of the use of the Sec secretory pathway to export heterologous proteins in a S. rimosus host. We aimed to develop a genetic tool kit for S. rimosus and to evaluate the extracellular production of target heterologous proteins of this industrial host. This study demonstrates that S. rimosus can produce the industrially important enzyme phytase AppA extracellularly, and analogous to E. coli as a host, application of His-Tag/Ni-affinity chromatography provides a simple and rapid approach to purify active phytase AppA in S. rimosus. We thus demonstrate that S. rimosus can be used as a potential alternative protein expression system.

Language:English
Keywords:Streptomyces rimosus, heterologous protein production, gene expression, gene tools, reporter system, phytase
Work type:Scientific work (r2)
Tipology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Number of pages:str. 2607-2620
Numbering:Vol. 102, iss. 6
UDC:602.3+602.6+604.4:579.873.7
ISSN on article:0175-7598
DOI:10.1007/s00253-018-8793-z Link is opened in a new window
COBISS.SI-ID:4879480 Link is opened in a new window
Views:199
Downloads:124
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Record is a part of a journal

Title:Applied microbiology and biotechnology
Shortened title:Appl. microbiol. biotechnol.
Publisher:Springer
COBISS.SI-ID:5136135 New window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Funding Programme:Raziskovalni projekti - podokrtorski
Project no.:C3330-17-529038
Name:Raziskovalci-2.0-UL-BF-529038
Acronym:
Project ID:info:eu-repo/grantAgreement/ARRS/Raziskovalni%20projekti%20-%20podokrtorski/C3330-17-529038

Secondary language

Language:Slovenian
Keywords:streptomicete, Streptomyces rimosus, sekundarni metaboliti, heterologni proteini, izražanje genov, encimi, fitaze

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