This master's thesis discuss the impact of the global financial and economic crisis, that emerged in 2007 in the United States and immediately globalized across all continents, to the functioning of non-governmental organizations. It represents a pressing problem of the modern world, wherefore non-governmental organizations are more active in times of various crises. The main goal of the master's thesis is to find out how the global financial and economic crisis affected non-governmental organization’s Greenpeace financing and functioning and furthermore with what kind of challenges are non-governmental organizations encountered these days. The main methods of research used are the method of data acquisition, statistical method and comparative analysis. The results of the analysis show that the period preceding the onset of the global financial and economic crisis (from 2005 to 2007) was marked by a surplus of the non-governmental organization Greenpeace. In the period between the global financial and economic crisis (from 2008 to 2009) the surplus reduced. The period after the onset of the global financial and economic crisis (from 2010 to 2015) was marked by a constant fluctuation in the surplus. On the basis of the findings non-governmental organization Greenpeace due to the impact of the global financial and economic crisis (from 2008 to 2009) recorded a decline in subsidies, grants, regressions, compensations and other revenues, wherefore devoted less financial resources to global environmental activities.