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Adhezija bakterije Streptococcus mutans na različne dentalne površine in zdravstveno vzgojna vloga medicinske sestre pri ustni higieni : diplomsko delo
Virant, Petra (Avtor), Zore, Anamarija (Mentor) Več o mentorju... Povezava se odpre v novem oknu, Bohinc, Klemen (Komentor)

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Izvleček
Uvod: Zobni karies je zelo razširjena kronična bolezen, ki je povezana z neustrezno ustno higieno in prehranskimi navadami. Karies nastane kot posledica tvorbe biofilma, v katerem prevladuje bakterija Streptococcus mutans. Na razvoj biofilma lahko vplivajo številni okoljski dejavniki in lastnosti materialov. Namen: dela je ugotoviti, kako različni dejavniki vplivajo na razvoj biofilma Streptococcus mutans na različnih dentalnih površinah. Metode dela: Pripravili smo si vzorce dentalnih površin, velikosti 1 cm2 in nekaj zob. Izbrali smo materiale, ki se uporabljajo v zobozdravstvu: amalgam, zlitina krom-kobalt, zlato, Chromasit, svetlobno polimerizirajoč kompozit in keramika. Najprej smo preučevali površino dentalnih materialov in zob. Vsem preučevanim dentalnim površinam smo merili hrapavost, zeta potencial in kontaktne kote. Za ugotavljanje adhezije smo sterilne dentalne ploščice najprej pustili 1 uro v umetni slini, nato smo jih prestavili v gojišče s kulturo bakterije Streptococcus mutans in inkubirali 10 ur. Po desetih urah smo pritrjene bakterije fiksirali in vzorce pregledali z vrstičnim elektronskim mikroskopom. Oprijete bakterije na mikrografu SEM smo ročno prešteli in rezultate podali v razmerju pokritosti dentalne površine z bakterijo in celotno površino. Iz literature je razvidno, da pH sline vpliva na adhezijo bakterije Streptococcus mutans, zato smo želeli preveriti nihanje pH v slini prostovoljcev in ugotoviti ali so nihanja res pomembna. Rezultati: Ugotovili smo, da se je največ bakterij oprijelo na zobno površino in najmanj na amalgam. pH vrednosti se niso bistveno spremenile. Razprava in sklep: V naši študiji smo ugotovili, da se bakterija Streptococcus mutans bolje adherira na zobno površino, kot na ostale površine. V literaturi je navedeno, da se z večanjem hrapavosti površin veča tudi adhezija, kar v naši študiji nismo dokazali. Iz literature je razvidno, da na razvoj biofilma bolj kot lastnosti materialov, vpliva koncentracija sladkorjev v hrani. Ob ugotavljanju nihanja pH v odvisnosti od zaužite sladkorne raztopine nismo zaznali pomembnih sprememb v pH, kar je lahko posledica premalo občutljive metode merjenja pH in premajhnega vzorca pregledanih oseb.

Jezik:Slovenski jezik
Ključne besede:S. mutans, adhezija bakterij, vplivi na adhezijo, preventiva
Vrsta gradiva:Diplomsko delo/naloga (mb11)
Organizacija:ZF - Zdravstvena fakulteta
Leto izida:2017
COBISS.SI-ID:5331051 Povezava se odpre v novem oknu
Število ogledov:36
Število prenosov:65
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
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Sekundarni jezik

Jezik:Angleški jezik
Naslov:Adhesion of the bacteria Streptococcus mutans to different dental surfaces and health educational role of nurse in oral hygiene : diploma thesis
Izvleček:
Introduction: Dental caries is a widespread chronic disease that is associated with inadequate oral hygiene and dietary habits. On dental surfaces a biofilm is formed, which later develops into caries. Caries occurs because of the formation of a biofilm in which Streptococcus mutans is predominant. The development of biofilm is influenced by many environmental factors and properties of materials. Purpose: To determine how different factors influence the development of Streptococcus mutans biofilm on different dental surfaces, which were studied. Methods: Samples of dental surfaces and few teeth were prepared. We selected materials used in dentistry: amalgam, chromium-cobalt alloy, gold, Chromasit, resin composite and ceramics. Firstly, surfaces of dental materials and teeth were studied. Surface roughness, zeta potential and contact angle of dental materials were measured. For determination of bacterial adhesion, sterile dental specimens and extracted teeth were immersed with an artificial saliva for an hour and then transferred to Petri dish; culture of Streptococcus mutans with broth was added and incubated for 10 hours. After 10 hours, the attached bacteria were fixed and the samples were examined with a scanning electron microscope. The adhered bacteria were manually counted and the results were given in percentage of the coverage of the dental surface with the bacteria. According to literature, the pH of saliva affects adhesion of Streptococcus mutans, so the fluctuation of pH in saliva was checked at volunteers and to determine if fluctuations really matter. Results: A significant higher number of bacteria was adhered to tooth surface than on other dental surfaces. The lowest number of adhered bacteria was found on amalgam surface. The pH values did not change significantly. Discussion and conclusion: It was found that Streptococcus mutans better adheres to the tooth surface than to the rest. The literature states that increasing surface roughness also increases adhesion, which was not proven in our study. It is evident from literature that development of biofilm is more influenced by the concentration of sugars in food than properties of materials. No significant changes in pH were noticed in the determination of the pH fluctuation due to the ingestion of the sugar solution. This may also be the result of the insufficiently sensitive method of pH measurement and small sample of the examined persons.

Ključne besede:S. mutans, bacterial adhesion, effects on adhesion, prevention

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