The purpose of the research was to evaluate the nutrient intakes of 20 students (12 female, 8 male) involved in master study program Nutrition and to compare the results with the recommendations, provided by the Guidelines of healthy nutrition for students. In the first part of the study the nutritional value of consumed meals was evaluated by means of 7-days dietary records, managed by students with the web tool OPEN. It was found out, that student's diet is not balanced as the students rarely included foods from all food groups, especially fruits, vegetables and fish, and they did not consume enough fluids. Among meals, students most often ate lunch and dinner, followed by snack and breakfast. The average daily energy intake was too low and did not meet the recommendations. The average energy ratios of nutrients (protein 18 %, fat 34 %, and total carbohydrates 48 % of the daily energy intake) were not in accordance with the guidelines. The intake of simple sugars was too high, and accounted for 15 % of the daily energy intake, instead of the recommended 10 %. In the second part of the study the nutritional value of the selected lunch of each student was determined with chemical analysis as well as by using the web tool OPEN and the results obtained through both methods were compared. The chemical analysis of lunches comprised the analysis of water, ash, protein, fat and dietary fibre content. From the analytical data the content of carbohydrates, energy value, nutrients energy ratios and energy density were calculated. When comparing the results regarding the source of data (chemical analysis, OPEN) it was found out that the nutrient contents in lunches, determined with chemical analysis were generally lower than values obtained with the web tool OPEN. With statistical analysis no significant differences regarding the method used were found, only the differences between the intake of some nutrients between male and female students were observed.