Preučevanje toksikološkega profila izbranih nanodelcev na celični liniji HepG2

Cundrič, Sandra (author), Žgur Bertok, Darja (mentor), Žegura, Bojana (co-mentor)

Nanodelci imajo velik potencial za uporabo v medicini in farmaciji, predvsem kot sistemi za dostavo zdravilnih učinkovin za boj proti rakavim obolenjem. Prednost je, da izbrano učinkovino/spojino lahko zaščitijo, stabilizirajo in selektivno dostavijo na tarčno mesto. Vendar je pred uporabo nanodelcev potrebno opredeliti njihovo toksično delovanje. Cilj magistrske naloge je bil preveriti morebiten toksičen učinek s poli-glutaminsko kislino (PGA) obdanih nanodelcev (ND) poli-epsilon- kaprolaktona (PCL), ND poli-(mlečne-ko-glikolne) kisline (PLGA) z inkapsuliranimi ND selena (Se) ter z govejim serumskim albuminom (BSA) obdanih ND Se. Po izpostavitvi celic HepG2 raztopini testiranih ND, smo s testom MTT določali citotoksičnost vzorcev, s testom DCFH-DA merili nastanek reaktivnih kisikovih spojin (ROS) in s tem vpliv na oksidativni stres, ter s testom komet preučevali genotoksično delovanje ND. Ugotovili smo, da noben od testiranih vzorcev ne izkazuje toksičnih učinkov na celicah človeškega hepatoma (HepG2). Največji porast ROS in prelomov DNA smo opazili pri celicah, ki so bile izpostavljene ND PCL brez PGA.

• Language: Slovenian
• Keywords: nanodelci, PCL, PLGA, Se, citotoksičnost, ROS, genotoksičnost, HepG2
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [S. Cundrič]
• Year: 2017
• PID: 20.500.12556/RUL-91989
• UDC: 606:61:57.083.36(043.2)
• COBISS.SI-ID: 8717945
• Publication date in RUL: 05.05.2017

Secondary language

• Language: English
• Title: Study of toxicological profile of selected nanoparticles on cell line HepG2

Abstract

Nanoparticles have a high potential for use in medicine and pharmacy, especially as nanodelivery systems used in anti-cancer therapy. Their advantage is protection, stabilization and selective delivery of a compound to a target site. Although, potential toxicity of nanoparticles need to be evaluated before their use. The goal of our master thesis was to investigate potential toxic effect of poly-epsilon-caprolactone (PCL) nanoparticles coated with poly-glutamic acid (PGA), poly-(lactic-co-glycolic) acid (PLGA) with encapsulated selenium (Se) nanoparticles and selenium (Se) nanoparticles coated with bovine serum albumin (BSA). After exposure to solutions of tested nanoparticles we evaluated their cytotoxicity with MTT assay, reactive oxygen species (ROS) production and subsequent oxidative stress with DCFH-DA assay and genotoxic effects of nanoparticles with Comet assay. Our results showed no biologically relevant toxic effects on human hepatoma (HepG2) cell line in all tested samples of nanoparticles although slightly increased ROS production and DNA strand breaks was detected in cells, which were exposed to ND PCL without PGA.

• Keywords: nanoparticles, PCL, PLGA, Se, cytotoxicity, ROS, genotoxicity, HepG2