Merjenje dihanja mikroorganizmov v remediiranih in originalnih vzorcih tal po metodi SIR

Pregelj, Žiga (avtor), Leštan, Domen (mentor)

Glavni cilj magistrske naloge je bilo ugotoviti razlike v hitrosti respiracije pred in po remediaciji tal z metodo SIR (substratno inducirana respiracija). Originalna tla iz Podkloštra in Mežiške doline so bila onesnažena s PTE (potencialno toksičnimi elementi) Pb, Zn in Cd. Remediacija je potekala na pilotni napravi z izpiranjem tal z EDTA (etilendiamin-tetraocetno kislino) 80 mmol EDTA na kg suhe snovi tal pri tleh iz Mežiške doline in 10 mmol EDTA na kg suhe snovi pri tleh iz Podkloštra. Primerjali smo respiracijo remediiranih in originalnih vzorcev pri induciranju respiracije tal s štirimi substrati (glukoza, škrob, mikroceluloza in kalčki lucerne). Poskus z vsakim substratom je trajal pet dni, razen pri glukozi, kjer je poskus trajal 24 ur. Zanimal nas je tudi vpliv remediacije na sposobnost tal za razgradnjo organskih snovi. V mežiških tleh je prišlo do razlike v hitrosti porabe substrata med originalnimi in remediiranimi tlemi. V zadnjem kinetičnem območju je bila v originalnih tleh hitrost porabe substrata večja pri glukozi (0,115 µmol O2/g SS na uro), škrobu (0,053 µmol O2/g SS) in kalčkih lucerne (0,643 mikro mol O2/g SS). Pri dodajanju mikroceluloze je prišlo do večje porabe kisika v remediiranih tleh (0,298 mikro mol O2/g SS). V tleh iz Podkloštra je bila poraba substrata hitrejša v remediiranih tleh: glukoza (0,104 mikro mol O2/g SS), škrob (0,150 mikro mol O2/g SS), mikroceluloza (0,186 mikro mol O2/g SS), kalčki lucerne (0,156 mikro mol O2/g SS). To pripisujemo različni kemijski sestavi tal in manjši koncentraciji liganda pri izpiranju tal ter odstranitvi PTE. Navedena razloga in nepojasnjeni del je v remediiranih tleh iz Podkloštra ugodno vplivalo na povečano respiracijo mikroorganizmov v primerjavi z remediiranimi tlemi iz Mežiške doline.

• Jezik: Slovenski jezik
• Ključne besede: SIR, respiracija, remediacija, dihanje tal, poraba organske snovi, mikroorganizmi
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Založnik: [Ž. Pregelj]
• Leto izida: 2017
• PID: 20.500.12556/RUL-91858
• UDK: 631.433.3:631.453(043.2)
• COBISS.SI-ID: 8712569
• Datum objave v RUL: 25.04.2017

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Measuring respiration of microorganisms in remediated and original soil with SIR metod

Izvleček

Soil respiration was measured with SIR (substrate induced respiration) method in remediated and original soil from Austria (Arnoldstein) and Slovenia (Mežica Valley). The original soil was contaminated with PTE (potentially toxic elements), such as Pb, Zn and Cd. Remediation was carried out on a pilot remediation facility which helped washing the soil with EDTA (ethylene diamine tetraacetic acid). 80 mmol of EDTA per kg of dry matter was used for the soil of the Mežica valley and 10 mM EDTA per kg of dry matter was used for the Austrian soil. Remediated and original soil samples were compared by adding four substrates (glucose, starch, microcelulose and alfalfa sprouts) to each of them. Different substrates were sequentially added to the original soil (in order written previously) every five days. In the soil of Mežica Valley, the discrepancy can be explained by the consumption rate of the substrate between the original and the remediated soil in the last kinetic zone: glucose (0.115 µmol O2/g DM/h), starch (0,053 µmol O2/g DM/h) and alfaalfa sprouts (0.643 µmol O2/g DM/h more in original soil). Regarding microcelulose substrate, there was a greater consumption of oxygen in the remediated soil (0.298 µmol O2/g DM/h). The soil from Austria had an opposite trend. In all cases, there was a greater respiration in remediated soil: glucose (0,104 µmol O2/g DM/h), starch (0,150 µmol O2/g DM/h) mikrocelulose (0,186 µmol O2/g DM/h), alfaalfa sprouts (0,156 µmol O2/g DM/h more in the remediated soil). This is attributed to the different chemical structure of the soil and reduction of the concentration of ligand for soil rinsing and removal of PTE, which had a favorable impact on the functioning of microorganisms.

• Ključne besede: SIR, soil remediation, soil respiration, organic matter uptake, microorganisms