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Catalog of MicroRNA seed polymorphisms in vertebrates
Zorc, Minja (Avtor), Jevšinek Skok, Daša (Avtor), Godnič, Irena (Avtor), Calin, George Adrian (Avtor), Horvat, Simon (Avtor), Jiang, Zhihua (Avtor), Dovč, Peter (Avtor), Kunej, Tanja (Avtor)

URLURL - Predstavitvena datoteka, za dostop obiščite http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0030737 Povezava se odpre v novem oknu

Izvleček
MicroRNAs (miRNAs) are a class of non-coding RNA that plays an important role in posttransriptional regulation of mRNA. Evidence has shown that miRNA gene variability might interfere with its function resulting in phenotypic variation and disease susceptibility. A major role in miRNA target recognition is ascribed to complementarity with the miRNA sees region that can be affected by polymorphisms. In the present study, we devloped an online tool for the detection of miRNA polymorphisms (miRNA SNiPer) in vertebrates (http://www.integratomics-time.com/miRNA-SNiPer) and generated a catalog of miRNA seed region polymorphisms (miR-seed-SNPs) consisting of 149 SNPs in six species. Although a majority of detected polymorhisms were due to point mutations, two consecutive nucleotide substitutions (double nucleotide polymorphisms, DNPs) were also identified in nine miRNAs. We determined that miR-SNPs are frequently located within the quantitative trait loci (QTL), chromosome fragile sites, and cancer susceptibility loci, indicating their potential role in the genetic control of various complex traits. To test this further, we performed an association abnalysis between the mmu-miR-717 seed SNP rs30372501, which is polymorphic in a large number of standard inbreed strains, and all phenotypic traits in these strains deposited in the Mouse Phenome Database. Analysis showed a significant association between the mmu-miR-717 seed SNP and a diverse array of traits including behavior, blood-clinical chemistry, body weight size and growth, and immune system suggesting that seed SNps can indeed have major pleiotropic effects. The bioinformatics analyses, data and tools developed in the present study can serve researchers as a starting point in testing more targeted hypotheses and designing experiments using optimal species or strains for further mechanistic studies.

Jezik:Angleški jezik
Ključne besede:molekularna genetika, mikro RNK, polimorfizem, bioinformatika, vretenčarji
Vrsta gradiva:Delo ni kategorizirano (r6)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:BF - Biotehniška fakulteta
Leto izida:2012
Št. strani:str. 1-8, e30737
Številčenje:Vol. 7, no. 1
UDK:575
ISSN pri članku:1932-6203
DOI:10.1371/journal.pone.0030737 Povezava se odpre v novem oknu
COBISS.SI-ID:3003528 Povezava se odpre v novem oknu
Število ogledov:646
Število prenosov:409
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
 
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Naslov:PloS one
Založnik:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 Povezava se odpre v novem oknu

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