The neuronal splicing factor Nova co-localizes with target RNAs in the dendrite

Racca, Claudia (author), Ule, Jernej (author)

Nova proteins are neuron-specifi c RNA binding proteins targeted by autoantibodies in a disorder manifest by failure of motor inhibition, and they regulate splicing and alternative 3 processing. Nova regulates splicing of RNAs encoding synaptic proteins, including the inhibitory glycine receptor f 2 subunit (GlyRf 2), and binds to others, including the GIRK2 channel. We found that Nova harbors functional NES and NLS elements, shuttles between the nucleus and cytoplasm, and that 50% of the protein localizes to the soma-dendritic compartment. Immunofl uoresence and EM analysis of spinal cord motor neurons demonstrated that Nova co-localizes beneath synaptic contacts indendrites with the same RNA, GlyRf 2, whose splicing it regulates in the nucleus. HITS-CLIP identifi ed intronic and 3 UTR sites where Nova binds to GlyRf 2 and GIRK2 transcripts in the brain. This led directly to the identifi cation of a 3 UTR localization element that mediates Nova-dependent localization of GIRK2 in primary neurons. These data demonstrate that HITS-CLIP can identify functional RNA localization elements, and they suggest new links between the regulation of nuclear RNA processing and mRNA localization.

• Language: English
• Keywords: RNK, beljakovine, nevroni, hrbtenjača
• Work type: Not categorized
• Typology: 1.01 - Original Scientific Article
• Organization: FDV - Faculty of Social Sciences
• Year: 2010
• Number of pages: Str. 1-16
• Numbering: Vol. 4, art. 5
• PID: 20.500.12556/RUL-33303
• UDC: 577.2
• ISSN on article: 1662-5110
• DOI: 10.3389/neuro.04.005.2010
• COBISS.SI-ID: 30083165
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Frontiers in neural circuits
• Shortened title: Front. neural circuits
• Publisher: Frontiers Research Foundation
• ISSN: 1662-5110
• COBISS.SI-ID: 30082909